氟烷可增强大鼠脑皮质切片中[3H]-乙酰胆碱的胞吐作用，而不增加钙内流。

Halothane enhances exocytosis of [3H]-acetylcholine without increasing calcium influx in rat brain cortical slices.

Gomez R S, Prado M A, Carazza F, Gomez M V

Departamento de Cirurgia, Sala 4000 Faculdade de Medicina da UFMG, Belo Horizonte-Minas Gerais-Brasil.

Br J Pharmacol. 1999 Jun;127(3):679-84. doi: 10.1038/sj.bjp.0702603.

Abstract

The effect of halothane on the release of [3H]-acetylcholine ([3H]-ACh) in rat brain cortical slices was investigated. 2. Halothane (0.018 mM) did not significantly affect the basal and the electrical field stimulation induced release of [3H]-ACh. However, halothane (0.063 mM) significantly increased the basal release of [3H]-ACh and this effect was additive with the electrical field stimulation induced release of [3H]-ACh. 3. The release of [3H]-ACh induced by 0.063 mM halothane was independent of the extracellular sodium and calcium ion concentration and was decreased by tetracaine, an inhibitor of Ca(2+)-release from intracellular stores or dantrolene, an inhibitor of Ca(2+)-release from ryanodine-sensitive stores 4. Using 2-(4-phenylpiperidino)-cyclohexanol (vesamicol), a drug that blocks the storage of ACh in synaptic vesicles, we investigated whether exocytosis of this neurotransmitter is involved in the effect of halothane. Vesamicol significantly decreased the release of [3H]-ACh evoked by halothane. 5. It is suggested that halothane may cause a Ca2+ release from intracellular stores that increases [3H]-ACh exocytosis in rat brain cortical slices.

摘要

研究了氟烷对大鼠脑皮质切片中[3H]-乙酰胆碱（[3H]-ACh）释放的影响。2. 氟烷（0.018 mM）对基础状态及电场刺激诱导的[3H]-ACh释放无显著影响。然而，氟烷（0.063 mM）显著增加了[3H]-ACh的基础释放，且此效应与电场刺激诱导的[3H]-ACh释放呈相加作用。3. 0.063 mM氟烷诱导的[3H]-ACh释放与细胞外钠和钙离子浓度无关，且可被四卡因（一种抑制细胞内钙库释放Ca(2+)的抑制剂）或丹曲林（一种抑制兰尼碱敏感钙库释放Ca(2+)的抑制剂）所降低。4. 使用2-(4-苯基哌啶基)-环己醇（维库溴铵），一种阻断ACh在突触小泡中储存的药物，我们研究了这种神经递质的胞吐作用是否参与氟烷的效应。维库溴铵显著降低了氟烷诱发的[3H]-ACh释放。5. 提示氟烷可能引起细胞内钙库释放Ca2+，从而增加大鼠脑皮质切片中[3H]-ACh的胞吐作用。