Life and Environment Science College, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, P.R. China.
Analyst. 2012 Dec 21;137(24):5716-9. doi: 10.1039/c2an35612k.
A convenient and environmentally friendly method of fabricating glucose biosensors is proposed. Glucose oxidase (GOD) was immobilized on electrochemically reduced graphene oxide (ERGO) which was adsorbed on the poly-L-lysine (PLL) modified glassy carbon electrode after being immersed in GO solution for 4 h. The electrochemical behaviors of GOD/ERGO/PLL/GC electrode have been investigated by cyclic voltammetry. Direct electron transfer between GOD immobilized with ERGO/PLL and GC electrode was observed. Moreover, the GOD/ERGO/PLL/GC electrode exhibited excellent electrocatalytic activity for the detection of glucose with a linear range from 0.25 to 5 mmol L(-1).
提出了一种方便且环保的葡萄糖生物传感器制造方法。将葡萄糖氧化酶（GOD）固定在电化学还原氧化石墨烯（ERGO）上，将其浸泡在 GO 溶液中 4 小时后吸附在聚-L-赖氨酸（PLL）修饰的玻碳电极上。通过循环伏安法研究了 GOD/ERGO/PLL/GC 电极的电化学行为。观察到固定在 ERGO/PLL 上的 GOD 与 GC 电极之间的直接电子转移。此外，GOD/ERGO/PLL/GC 电极对葡萄糖的检测表现出优异的电催化活性，线性范围为 0.25 至 5 mmol L(-1)。
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