Puchkov M N, Vassarais R A, Korepanova E A, Osipov A N
Department of Medical Biophysics, N.I. Pirogov Russian National Research Medical University, Moscow, Russia.
Biochim Biophys Acta. 2013 Feb;1828(2):208-12. doi: 10.1016/j.bbamem.2012.10.002. Epub 2012 Oct 19.
Interaction of cytochrome c with cardiolipin in the presence of hydrogen peroxide induces peroxidase activity in cytochrome c and the ability to oxidize membrane lipids. These cytochrome c properties play a substantial role in the cytochrome c-mediated apoptotic reactions. In the present study the electric properties (specific capacitance and integral conductance) of the cardiolipin-containing asolectin planar bilayer lipid membranes (pBLM) in the presence of cytochrome c and hydrogen peroxide were studied. Cytochrome c interaction with cardiolipin-containing pBLM in the presence of hydrogen peroxide resulted in the dramatic increase of the conductance, pore production, their growth up to 3.5 nm diameter and subsequent membrane destruction. In the absence of hydrogen peroxide cytochrome c demonstrated almost no effect on the membrane capacitance and conductance. The data obtained prove the pivotal role of cytochrome c and membrane lipids in the permeabilization of pBLM. Correlation of apoptotic reactions and cytochrome c-mediated membrane permeability is discussed.
在过氧化氢存在的情况下，细胞色素c与心磷脂的相互作用会诱导细胞色素c产生过氧化物酶活性以及氧化膜脂的能力。这些细胞色素c的特性在细胞色素c介导的凋亡反应中起着重要作用。在本研究中，研究了在细胞色素c和过氧化氢存在的情况下，含心磷脂的大豆卵磷脂平面双层脂质膜（pBLM）的电学性质（比电容和积分电导）。在过氧化氢存在的情况下，细胞色素c与含心磷脂的pBLM相互作用导致电导急剧增加、形成孔道，孔道直径增长至3.5 nm并随后导致膜破坏。在没有过氧化氢的情况下，细胞色素c对膜电容和电导几乎没有影响。所获得的数据证明了细胞色素c和膜脂在pBLM通透化过程中的关键作用。讨论了凋亡反应与细胞色素c介导的膜通透性之间的相关性。
