Department of Physics, Kansas State University, Manhattan, KS 66506-2601, USA.
Nanotechnology. 2012 Nov 16;23(45):455105. doi: 10.1088/0957-4484/23/45/455105. Epub 2012 Oct 19.
Maneuverable, high aspect ratio poly(3,4-ethylene dioxythiophene) (PEDOT) fibers are fabricated for use as cellular force probes that can interface with individual pseudopod adhesive contact sites without forming unintentional secondary contacts to the cell. The straight fibers have lengths between 5 and 40 μm and spring constants in the 0.07-23.2 nN μm(-1) range. The spring constants of these fibers were measured directly using an atomic force microscope (AFM). These AFM measurements corroborate determinations based on the transverse vibrational resonance frequencies of the fibers, which is a more convenient method. These fibers are employed to characterize the time dependent forces exerted at adhesive contacts between apical pseudopods of highly migratory D. discoideum cells and the PEDOT fibers, finding an average terminal force of 3.1 ± 2.7 nN and lifetime of 23.4 ± 18.5 s to be associated with these contacts.
可操纵的、高纵横比的聚（3,4-亚乙基二氧噻吩）（PEDOT）纤维被制造出来用作细胞力探针，可与单个伪足黏附接触点进行接口，而不会与细胞形成无意的二次接触。这些直纤维的长度在 5 到 40 微米之间，其弹簧常数在 0.07 到 23.2 nN μm(-1)的范围内。这些纤维的弹簧常数是使用原子力显微镜（AFM）直接测量的。这些 AFM 测量结果与基于纤维的横向振动共振频率的测定结果相符，这是一种更方便的方法。这些纤维用于表征高度迁移的 D. discoideum 细胞的顶伪足和 PEDOT 纤维之间的黏附接触处的时间依赖性力，发现与这些接触相关的平均末端力为 3.1 ± 2.7 nN，寿命为 23.4 ± 18.5 s。
