Department of Mechanical Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia.
Nanotechnology. 2012 Nov 16;23(45):455106. doi: 10.1088/0957-4484/23/45/455106. Epub 2012 Oct 19.
The effect of the recently developed graphene nanoflakes (GNFs) on the polymerase chain reaction (PCR) has been investigated in this paper. The rationale behind the use of GNFs is their unique physical and thermal properties. Experiments show that GNFs can enhance the thermal conductivity of base fluids and results also revealed that GNFs are a potential enhancer of PCR efficiency; moreover, the PCR enhancements are strongly dependent on GNF concentration. It was found that GNFs yield DNA product equivalent to positive control with up to 65% reduction in the PCR cycles. It was also observed that the PCR yield is dependent on the GNF size, wherein the surface area increases and augments thermal conductivity. Computational fluid dynamics (CFD) simulations were performed to analyze the heat transfer through the PCR tube model in the presence and absence of GNFs. The results suggest that the superior thermal conductivity effect of GNFs may be the main cause of the PCR enhancement.
本文研究了最近开发的石墨烯纳米薄片（GNFs）对聚合酶链反应（PCR）的影响。使用 GNFs 的基本原理是其独特的物理和热性能。实验表明，GNFs 可以提高基液的导热率，结果还表明 GNFs 是提高 PCR 效率的潜在增强剂；此外，PCR 增强强烈依赖于 GNF 浓度。研究发现，GNFs 可产生与阳性对照相当的 DNA 产物，PCR 循环次数减少了 65%。还观察到 PCR 产量取决于 GNF 的尺寸，其中表面积增加并提高了导热率。进行了计算流体动力学（CFD）模拟，以分析在存在和不存在 GNFs 的情况下通过 PCR 管模型的传热。结果表明，GNFs 的卓越导热效应可能是 PCR 增强的主要原因。
