Winchester Engineering and Analytical Center, Winchester, MA, USA.
Eye Contact Lens. 2012 Nov;38(6):379-84. doi: 10.1097/ICL.0b013e31826f1004.
To investigate the effects of eight different soft contact lenses on disinfection efficacy of a multipurpose solution (MPS) containing polyhexamethylene biguanide (PHMB) against Fusarium solani.
Six silicone hydrogel lenses (galyfilcon A, senofilcon A, comfilcon A, enfilcon A, balafilcon A, and lotrifilcon B) and two conventional hydrogel lenses (polymacon and etafilcon A) were placed in polypropylene lens cases filled with MPS containing 0.0001% PHMB and soaked for 6, 12, 24, 72, and 168 hours. After each interval, depleted MPS from lens cases were removed and assayed for activity against F. solani according to International Organization for Standardization (ISO) 14729 stand-alone procedure. A portion was aliquoted for chemical analysis.
Soaking etafilcon A, balafilcon A, and polymacon lenses for 6 hours reduced the concentration of PHMB in MPS by more than half the stated labeled concentration, with concentrations below the limit of detection for etafilcon A-depleted and balafilcon A-depleted solutions after 12 and 72 hours of soaking, respectively. Except for comfilcon A-depleted solutions, all others failed to consistently obtain one log reduction of F. solani. The solutions soaked with etafilcon A, balafilcon A, and polymacon lenses for 24 hours or more lost all or almost all fungicidal activity against F. solani.
Over time, the disinfectant uptake by some lenses can significantly reduce the PHMB concentration and the fungicidal activity of the MPS against F. solani. Current ISO methodology does not address the reduction in microbiocidal efficacy when lenses are soaked in MPS. The ISO committee should consider adding "soaking experiments" to quantify the effect that contact lens materials have on the performance of MPSs.
研究 8 种不同软性隐形眼镜对含有聚六亚甲基双胍（PHMB）的多功能溶液（MPS）对茄病镰刀菌消毒效果的影响。
将 6 种硅水凝胶镜片（加利福康 A、新福康 A、康氟康 A、恩氟康 A、巴氟康 A 和 lotrafilcon B）和 2 种传统水凝胶镜片（聚甲基丙烯酸羟乙酯和埃氟康 A）放置在充满含有 0.0001% PHMB 的 MPS 的聚丙烯镜片盒中，并浸泡 6、12、24、72 和 168 小时。每个间隔后，从镜片盒中取出耗尽的 MPS 并根据国际标准化组织（ISO）14729 独立程序对 F. solani 的活性进行检测。一部分进行化学分析。
浸泡 etafilcon A、balafilcon A 和 polymacon 镜片 6 小时后，MPS 中的 PHMB 浓度降低了一半以上，低于 etafilcon A 耗尽和 balafilcon A 耗尽溶液的检测限 12 和 72 小时后分别浸泡。除了 comfilcon A 耗尽溶液外，其他所有溶液均未能对 F. solani 进行一致的对数减少。用 etafilcon A、balafilcon A 和 polymacon 镜片浸泡 24 小时或更长时间后，溶液对 F. solani 的杀菌活性完全丧失或几乎完全丧失。
随着时间的推移，一些镜片的消毒剂吸收会显著降低 MPS 中的 PHMB 浓度和对 F. solani 的杀菌活性。当前的 ISO 方法学没有解决镜片浸泡在 MPS 中时对微生物杀灭效果的降低。ISO 委员会应考虑添加“浸泡实验”来量化接触镜材料对 MPS 性能的影响。
