Rosenthal Ruth A, Dassanayake Nissanke L, Schlitzer Ronald L, Schlech Barry A, Meadows David L, Stone Ralph P
Alcon Research, Ltd., Fort Worth, TX 76134, USA.
Eye Contact Lens. 2006 Dec;32(6):262-6. doi: 10.1097/ICL.0b013e31802b413f.
With recent outbreaks of Fusarium keratitis related to contact lens wear, studies were conducted to determine the biocide uptake during lens storage, and the resulting effect on antifungal activity of related products.
ACUVUE 2 (etafilcon A) soft, hydrophilic contact lenses (group IV) were soaked from 1 hour to 7 days in OPTI-FREE Express and OPTI-FREE RepleniSH multipurpose disinfecting solutions with POLYQUAD (polyquaternium-1) and ALDOX (myristamidopropyl dimethylamine) biocides and multipurpose solutions, Bausch & Lomb ReNu with MoistureLoc (Alexidine), ReNu MultiPlus (polyhexamethylene biguanide [PHMB]), and AMO Complete MoisturePLUS (PHMB). Storage solutions were tested to evaluate the effect of preservative uptake on the residual biocide activity against Fusarium solani.
Approximately 30% to 60% of the PHMB and Alexidine were depleted by 6 hours, with comparable loss of antimicrobial activity. Decreasing activity was noted with corresponding decreases in active concentration throughout the course of the evaluation. The POLYQUAD systems retained nearly 100% of the biocide and fungicidal activity and maintained their concentration in the solution.
OPTI-FREE Express and OPTI-FREE RepleniSH multipurpose disinfecting solutions maintained fungicidal efficacy after storage of lenses. The Alexidine- and PHMB-based multipurpose solutions tested showed significant uptake of preservative into group IV lenses, resulting in a decrease in the residual activity of the storage solution. The POLYQUAD systems showed a low uptake of biocide and maintained fungicidal efficacy against F. solani.
鉴于近期与隐形眼镜佩戴相关的镰刀菌角膜炎爆发，开展了多项研究以确定镜片储存期间杀菌剂的摄取情况，以及由此对相关产品抗真菌活性产生的影响。
将ACUVUE 2（依他氟康A）软性亲水隐形眼镜（IV组）在含有POLYQUAD（聚季铵盐-1）和ALDOX（肉豆蔻酰胺丙基二甲胺）杀菌剂的OPTI-FREE Express和OPTI-FREE RepleniSH多功能消毒溶液以及多功能溶液中浸泡1小时至7天，这些多功能溶液包括博士伦的ReNu with MoistureLoc（洗必泰）、ReNu MultiPlus（聚六亚甲基双胍[PHMB]）和AMO Complete MoisturePLUS（PHMB）。对储存溶液进行测试，以评估防腐剂摄取对针对茄病镰刀菌的残留杀菌剂活性的影响。
约30%至(60%)的PHMB和洗必泰在6小时内耗尽，抗菌活性有相应损失。在整个评估过程中，随着活性浓度的相应降低，活性也在下降。POLYQUAD系统保留了近(100%)的杀菌剂和杀真菌活性，并在溶液中保持其浓度。
OPTI-FREE Express和OPTI-FREE RepleniSH多功能消毒溶液在镜片储存后保持了杀真菌效力。所测试的基于洗必泰和PHMB的多功能溶液显示防腐剂大量摄取到IV组镜片中，导致储存溶液的残留活性降低。POLYQUAD系统显示杀菌剂摄取量低，并对茄病镰刀菌保持杀真菌效力。
