Guo Lei, Xue Tian-Yang, Xu Wei, Gao Ji-Zhao
Department of Pediatrics, The Hospital Affiliated to Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2012 Oct;14(10):780-4.
To investigate the effects of matrine on the proliferation and apoptosis of human rhabdomyosarcoma RD cells in vitro, and to explore the mechanism of matrine inducing apoptosis of RD cells.
MTT assay was used to measure the proliferation inhibition rates of RD cells that were treated with matrine (final concentrations= 0.5, 1.0, 1.5, and 2.0 mg/mL). Flow cytometry was used to evaluate the apoptosis of RD cells treated with the four concentrations of matrine. RT-PCR was used to measure the mRNA expression of cyclin D1 and survivin in RD cells treated with 0.5, 1.0, and 1.5 mg/mL of matrine.
The RD cells treated with various concentrations of matrine showed significantly higher proliferation inhibition rates and apoptotic rates than those that were not treated with matrine (P<0.01), and with increased matrine concentration, the proliferation inhibition rate of RD cells increased gradually, thus exhibiting a dose dependence. The mRNA expression of cyclin D1 and survivin was seen in all RD cells, but was significantly lower in RD cells treated with matrine than in those that were not treated with matrine (P<0.01). There were significant differences in cyclin D1 mRNA level among the RD cells treated with 0.5, 1.0, and 1.5 mg/mL of matrine (P<0.05), while there was significant difference in survivin mRNA level between the RD cells treated with 0.5 and 1.5 mg/mL of matrine (P<0.05).
Matrine can significantly inhibit proliferation and induce apoptosis of RD cells, which may be related to downregulating the mRNA expression of cyclin D1 and survivin.
探讨苦参碱对人横纹肌肉瘤RD细胞体外增殖及凋亡的影响，并探讨苦参碱诱导RD细胞凋亡的机制。
采用MTT法检测苦参碱（终浓度=0.5、1.0、1.5和2.0mg/mL）处理后RD细胞的增殖抑制率。采用流式细胞术评估四种浓度苦参碱处理后RD细胞的凋亡情况。采用RT-PCR检测0.5、1.0和1.5mg/mL苦参碱处理后RD细胞中细胞周期蛋白D1和生存素的mRNA表达。
不同浓度苦参碱处理的RD细胞增殖抑制率和凋亡率均显著高于未处理的RD细胞（P<0.01），且随着苦参碱浓度的增加，RD细胞的增殖抑制率逐渐升高，呈剂量依赖性。所有RD细胞均可见细胞周期蛋白D1和生存素的mRNA表达，但苦参碱处理的RD细胞中其表达显著低于未处理的RD细胞（P<0.01）。0.5、1.0和1.5mg/mL苦参碱处理的RD细胞中细胞周期蛋白D1mRNA水平存在显著差异（P<0.05），而0.5和1.5mg/mL苦参碱处理的RD细胞中生存素mRNA水平存在显著差异（P<0.05）。
苦参碱可显著抑制RD细胞的增殖并诱导其凋亡，这可能与下调细胞周期蛋白D1和生存素的mRNA表达有关。
