[苦参碱和氧化苦参碱对SMMC - 7721细胞系中Stat3和Stat5增殖及表达的影响]

[Effect of matrine and oxymatrine on proliferation and expression of Stat3 and Stat5 in SMMC-7721 cell line].

作者信息

Zheng Yan-Min, Li Xuan, Zhao Hong-Yan, Zhao Jun-Yan

机构信息

Department of Gastroenterology, Affiliated Hospital, Medical Collage of Chinese People's Armed Police Forces, Tianjin 300162, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2008 Oct;33(19):2234-7.

PMID:19166015

Abstract

OBJECTIVE

To study the effect of matrine and oxymatrine on proliferation and the expression of Stat3, Stat5 mRNA in SMMC-7721 cell line.

METHOD

Treated with matrine and oxymatrine, the inhibitory effect on SMMC-7721 cell proliferation was detected by MTT, double fluorescence labeling was applied to measure the apotosis ratios of SMMC-7721cells, the expression of Stat3 and Stat5 mRNA in SMMC-7721 cell line were assessed with RT-PCR.

RESULT

Matrine and oxymatrine could inhibit the proliferation and induce the apoptosis of SMMC-7721 cells and it was time and dose dependent, the expression of Stat3 and Stat5 mRNA in SMMC-7721 cell with matrine and oxymatrine were significantly lower than those in control group (P<0.05 or P<0.01). Compared with the same dose of matrine and oxymatrine, matrine showed stronger effect on cell proliferation, apoptosis, and Stat3 and Stat5 mRNA (P<0.05 or P<0.01).

CONCLUSION

Matrine and oxymatrine inhibited the proliferation and induced the of SMMC-7721 cells significantly, the mechanism of which might be related to the down-regulation of stat3 and stat5 mRNA and inhibition of the signaling transduction pathway.

摘要

目的

研究苦参碱和氧化苦参碱对SMMC - 7721细胞系增殖及Stat3、Stat5 mRNA表达的影响。

方法

用苦参碱和氧化苦参碱处理，采用MTT法检测对SMMC - 7721细胞增殖的抑制作用，应用双荧光标记法检测SMMC - 7721细胞的凋亡率，用RT - PCR法检测SMMC - 7721细胞系中Stat3和Stat5 mRNA的表达。

结果

苦参碱和氧化苦参碱可抑制SMMC - 7721细胞的增殖并诱导其凋亡，呈时间和剂量依赖性，苦参碱和氧化苦参碱处理的SMMC - 7721细胞中Stat3和Stat5 mRNA的表达明显低于对照组（P<0.05或P<0.01）。与相同剂量的苦参碱和氧化苦参碱相比，苦参碱对细胞增殖、凋亡及Stat3和Stat5 mRNA的作用更强（P<0.05或P<0.01）。