Rajapakshage Bandula Kumara Wickramasekara, Yamasaki Masahiro, Murakami Masahiro, Tamura Yu, Lim Sue Yee, Osuga Tatsuyuki, Sasaki Noboru, Nakamura Kensuke, Ohta Hiroshi, Takiguchi Mitsuyoshi
Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Jpn J Vet Res. 2012 Aug;60(2-3):51-61.
In our previous study, the level of parasitemia of the diminazene aceturate (DA)-resistant B. gibsoni isolate was continuously lower than that of the wild-type, indicating the possible alteration of energy metabolism in that isolate. Therefore, in the present study, the concentrations of ATP, glucose, lactate, and pyruvate, and the activities of lactate dehydrogenase and pyruvate kinase in the wild-type and DA-resistant isolate of B. gibsoni were measured and compared to investigate the amount of energy generation and the activity of the glycolysis pathway. As a result, the intracellular ATP and glucose concentrations in the DA-resistant B. gibsoni isolate were significantly higher than those in the wild-type. Meanwhile, the concentrations of lactate and pyruvate and the activities of lactate dehydrogenase and pyruvate kinase in the DA-resistant B. gibsoni isolate were not different from those in the wild-type. These results indicated that the DA-resistant B. gibsoni isolate contained a higher ATP concentration than the wild-type, but the activity of the glycolysis pathway was not altered in the DA-resistant B. gibsoni isolate. However, we could not determine the mechanism of the high energy production of the DA-resistant B. gibsoni isolate. Further studies on the energy metabolism of B. gibsoni are necessary to clarify the mechanism of the high energy production in the DA-resistant B. gibsoni isolate.
在我们之前的研究中，对乙酰马啉（DA）耐药的吉氏巴贝斯虫分离株的虫血症水平持续低于野生型，这表明该分离株的能量代谢可能发生了改变。因此，在本研究中，我们测定并比较了吉氏巴贝斯虫野生型和DA耐药分离株中ATP、葡萄糖、乳酸和丙酮酸的浓度，以及乳酸脱氢酶和丙酮酸激酶的活性，以研究能量产生量和糖酵解途径的活性。结果显示，DA耐药的吉氏巴贝斯虫分离株细胞内的ATP和葡萄糖浓度显著高于野生型。同时，DA耐药的吉氏巴贝斯虫分离株中乳酸和丙酮酸的浓度以及乳酸脱氢酶和丙酮酸激酶的活性与野生型并无差异。这些结果表明，DA耐药的吉氏巴贝斯虫分离株所含的ATP浓度高于野生型，但DA耐药的吉氏巴贝斯虫分离株中糖酵解途径的活性并未改变。然而，我们无法确定DA耐药的吉氏巴贝斯虫分离株高能量产生的机制。有必要对吉氏巴贝斯虫的能量代谢进行进一步研究，以阐明DA耐药的吉氏巴贝斯虫分离株高能量产生的机制。
