黄体生成素释放激素类似物作为一种癌靶药物的单分子识别力谱研究。

Single molecular recognition force spectroscopy study of a luteinizing hormone-releasing hormone analogue as a carcinoma target drug.

机构信息

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China.

出版信息

J Phys Chem B. 2012 Nov 15;116(45):13331-7. doi: 10.1021/jp306882r. Epub 2012 Oct 31.

DOI:10.1021/jp306882r

PMID:23094688

Abstract

The luteinizing hormone-releasing hormone- Pseudomonas aeruginosa exotoxin 40 (LHRH-PE40), is a candidate target drug associated with elevated LHRH receptor (LHRH-R) expression in malignant tumor tissue. The capability of LHRH-PE40 to recognize LHRH-Rs on a living cell membrane was studied with single molecular recognition force spectroscopy (SMFS) based on atomic force microscopy (AFM). The recognition force of LHRH-PE40/LHRH-R was compared with that of LHRH/LHRH-R by dynamic force spectroscopy. Meanwhile, cell growth inhibition assay and fluorescence imaging were presented as complementary characterization. The results show that LHRH moiety keeps its capability to recognize LHRH-R specifically, which implies that recombinant protein LHRH-PE40 can be a promising target drug.

摘要

黄体生成素释放激素-铜绿假单胞菌外毒素 40（LHRH-PE40）是一种候选靶向药物，与恶性肿瘤组织中黄体生成素受体（LHRH-R）的表达升高有关。采用基于原子力显微镜（AFM）的单分子识别力谱（SMFS）研究了 LHRH-PE40 在活细胞膜上识别 LHRH-R 的能力。通过动态力谱比较了 LHRH-PE40/LHRH-R 的识别力。同时，还呈现了细胞生长抑制实验和荧光成像作为补充特征。结果表明，LHRH 部分保持其特异性识别 LHRH-R 的能力，这意味着重组蛋白 LHRH-PE40 可以成为一种有前途的靶向药物。