通过原子力显微镜评估抗癌药物西妥昔单抗的疗效。

Evaluating the efficacy of the anticancer drug cetuximab by atomic force microscopy.

作者信息

Zhang Qingrong, Shi Yan, Xu Haijiao, Zhou Lulu, Gao Jing, Jiang Junguang, Cai Mingjun, Shan Yuping

机构信息

School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology Changchun 130012 China

Changchun Institute of Applied Chemistry, State Key Laboratory of Electroanalytical Chemistry Chinese Academy of Science Renmin St 5625 Changchun Jilin130022 China

出版信息

RSC Adv. 2018 Jun 13;8(39):21793-21797. doi: 10.1039/c8ra03215g.

DOI:10.1039/c8ra03215g

PMID:35541738

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9081852/

Abstract

Cetuximab is a monoclonal antibody that binds to the epidermal growth factor receptor, which is important in the growth of many cancers. However, the biophysical characteristics of cetuximab as an anti-cancer drug remain elusive. In this study, we adopted atomic force microscopy to measure the mechanical properties of cancer cells following cetuximab treatment and the biomechanical properties of cetuximab and epidermal growth factor receptor interactions. Atomic force microscopy can be implemented as a platform for further investigations that target the cellular stiffness and affinity of ligand-receptor as a therapeutic choice.

摘要

西妥昔单抗是一种单克隆抗体，可与表皮生长因子受体结合，而表皮生长因子受体在许多癌症的生长过程中起着重要作用。然而，西妥昔单抗作为一种抗癌药物的生物物理特性仍然难以捉摸。在本研究中，我们采用原子力显微镜来测量西妥昔单抗治疗后癌细胞的力学性质以及西妥昔单抗与表皮生长因子受体相互作用的生物力学性质。原子力显微镜可作为一个平台用于进一步研究，这些研究以细胞硬度和配体 - 受体亲和力作为治疗选择为目标。