Institute of Wastewater Management and Water Protection, Hamburg University of Technology, Hamburg, Germany.
J Toxicol Environ Health A. 2012;75(22-23):1351-8. doi: 10.1080/15287394.2012.721167.
Cryptosporidium parvum is one of the most common human parasitic protozoa and is responsible for many waterborne outbreaks in several industrialized countries. The oocyst, which is the infective form, is known to be highly resistant to wastewater treatment procedures and represents a potential hazard to human populations through contaminated raw or treated wastewater. In this investigation, the occurrence of Cryptosporidium in wastewater samples was monitored and removal efficiency was assessed. Treated (effluent) and untreated (influent) wastewater samples were collected seasonally over a period of 2 years. Oocysts were repeatedly detected in influent and effluent samples collected from the treatment plant during all sampling seasons, with a mean concentration of 782 oocysts/L. The seasonal distribution showed that oocysts are predominant during autumn and winter. Molecular analyses via the small (18S) subunit of rRNA amplification and subsequent sequencing with an objective of characterizing the oocysts revealed that Cryptosporidium parvum was the dominant Cryptosporidium parasite present in wastewater.
微小隐孢子虫是最常见的人体寄生原生动物之一，也是导致几个工业化国家发生许多水源性暴发的原因。卵囊是感染的形式，已知对废水处理过程具有高度抗性，并通过受污染的原始或处理后的废水对人群构成潜在危害。在本研究中，监测了废水中隐孢子虫的发生情况，并评估了去除效率。在两年的时间里，季节性地采集处理过的（出水）和未处理的（进水）废水样本。在所有采样季节，从处理厂采集的进水和出水样本中反复检测到卵囊，平均浓度为 782 个卵囊/L。季节性分布表明，卵囊在秋季和冬季占优势。通过扩增小（18S）亚基 rRNA 并进行测序进行分子分析，以确定卵囊的特征，结果表明微小隐孢子虫是废水中主要存在的隐孢子虫寄生虫。
