Department of Genetics, Institute for Experimental Medicine, Istanbul University, Vakif Gureba Cad., 34080, Sehremini, Istanbul, Turkey.
Mol Biol Rep. 2012 Dec;39(12):10907-14. doi: 10.1007/s11033-012-1990-4. Epub 2012 Oct 25.
The aim of this study was to examine the relationship between APOA4 gene T347S polymorphism with obesity measures and serum lipids in Turkish adults. Randomly selected sample of 1,554 adults (754 men, mean age 50.4 ± 11.9 years and 800 women, mean age 49.6 ± 11.8 years) were included in the study. 346 Women (43.2 %) were postmenopausal. Genotyping was performed by using hybridization probes in real-time PCR. Not men but postmenopausal women, carrying the S347 allele, were associated with 1.5 kg/m(2) higher BMI (P = 0.016) and 3.6 cm wider waist circumference (P = 0.005) than postmenopausal T347 homozygotes, controlled for covariates. Logistic regression analyses of this polymorphism, adjusted for age, fasting triglyceride, smoking status, alcohol consumption and physical activity disclosed the rare allele to be associated with obesity in postmenopausal women at an odds of 1.80 (95 % CI 1.09-2.97; P = 0.021). Serum apoB level was lower in S347 allele carriers (110.9 ± 2.9 mg/dL) than in T347 homozygotes (119.0 ± 2.4 mg/dL; P = 0.035) in men but not women. APOA4 T347S polymorphism was unrelated to lipids and other lipoproteins in either gender. The APOA4 S347 allele predisposes to obesity and high waist circumference in Turkish postmenopausal women. ApoB levels are lower only in men in S347 allele carriers.
本研究旨在探讨 APOA4 基因 T347S 多态性与肥胖指标和血清脂质在土耳其成年人中的关系。研究纳入了 1554 名成年人（754 名男性，平均年龄 50.4 ± 11.9 岁，800 名女性，平均年龄 49.6 ± 11.8 岁）。346 名女性（43.2%）处于绝经后状态。采用实时 PCR 杂交探针进行基因分型。与绝经后 T347 纯合子相比，携带 S347 等位基因的绝经后女性不仅 BMI 增加 1.5kg/m²（P=0.016），而且腰围增加 3.6cm（P=0.005），但男性没有这种关联。对该多态性进行 logistic 回归分析，调整年龄、空腹甘油三酯、吸烟状态、饮酒和体力活动等因素后，发现该罕见等位基因与绝经后女性肥胖的发生相关，比值比为 1.80（95%可信区间 1.09-2.97；P=0.021）。与 T347 纯合子相比，携带 S347 等位基因的男性血清 apoB 水平较低（110.9 ± 2.9mg/dL），而非女性（119.0 ± 2.4mg/dL；P=0.035）。APOA4 T347S 多态性与两性的血脂和其他脂蛋白无关。APOA4 S347 等位基因易导致土耳其绝经后女性肥胖和高腰围。仅在男性中，S347 等位基因携带者的 apoB 水平较低。
