Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.
Mol Biol Rep. 2013 Feb;40(2):1227-39. doi: 10.1007/s11033-012-2165-z. Epub 2012 Oct 25.
A full-length cDNA of 1,537 nucleotides was cloned from Medicago sativa L. cv. "Zhongmu No. 1" by rapid amplification of cDNA ends. It was designated as MsZIP, encoding a protein of 340 amino acids. The protein molecular weight was 36.43 kDa, and the theoretical isoelectric point was 5.72. The MsZIP preferentially localized in nucleus and have signal peptide. Blast analysis revealed that MsZIP shared the highest homology with some bZIP proteins of M. truncatula. The transcript of MsZIP was strongly enriched in leaf compared with root and stem of mature alfalfa plants. MsZIP was strongly induced by 15 % PEG6000 (polyethylene glycol), 50 μM abscisic acid, 200 mM NaCl, 70 μM gibberellic acid, 5 mM salicylic acid and 200 μM methyl jasmonate. Physiological resistance parameters were measured in the transgenic tobacco. Malondialdehyde content, relative water content, soluble sugar content, soluble protein content and proline content in transgenic tobacco increased compared with non-transgenic tobacco under salt stress or drought stress. The results showed that accumulation of the MsZIP protein in the vegetative tissues of transgenic plants enhanced their tolerance to osmotic pressure stress. These results demonstrate a role for the MsZIP protein in stress protection and suggest the potential of the MsZIP gene for genetic engineering of salt tolerance and drought tolerance.
从紫花苜蓿 cv. "中苜 1 号"克隆全长 cDNA 1537 个核苷酸。它被命名为 MsZIP，编码 340 个氨基酸的蛋白质。该蛋白分子量为 36.43kDa，理论等电点为 5.72。MsZIP 优先定位于核内，并具有信号肽。Blast 分析表明，MsZIP 与某些 M. truncatula 的 bZIP 蛋白具有最高的同源性。MsZIP 的转录在成熟苜蓿植物的叶片中比根和茎中强烈富集。MsZIP 强烈诱导 15%PEG6000(聚乙二醇)、50μM 脱落酸、200mM NaCl、70μM 赤霉素、5mM 水杨酸和 200μM 茉莉酸甲酯。在转基因烟草中测量了生理抗性参数。与非转基因烟草相比，盐胁迫或干旱胁迫下转基因烟草的丙二醛含量、相对含水量、可溶性糖含量、可溶性蛋白含量和脯氨酸含量增加。结果表明，MsZIP 蛋白在转基因植物营养组织中的积累增强了它们对渗透压胁迫的耐受性。这些结果表明 MsZIP 蛋白在应激保护中的作用，并表明 MsZIP 基因在耐盐性和耐旱性遗传工程中的潜力。
