Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University), Ministry of Education, 26 Hexing Road, Harbin 150040, PR China.
J Plant Physiol. 2010 Feb 15;167(3):222-30. doi: 10.1016/j.jplph.2009.09.008. Epub 2009 Oct 23.
Basic leucine zipper proteins (bZIPs) are transcription factors that bind abscisic acid (ABA)-responsive elements (ABREs) and enable plants to withstand adverse environmental conditions. In the present study, a novel bZIP gene, ThbZIP1 was cloned from Tamarix hispida. Expression studies in T. hispida showed differential regulation of ThbZIP1 in response to treatment with NaCl, polyethylene glycol (PEG) 6000, NaHCO(3), and CdCl(2), suggesting that ThbZIP1 is involved in abiotic stress responses. To identify the physiological responses mediated by ThbZIP1, transgenic tobacco plants overexpressing exogenous ThbZIP1 were generated. Various physiological parameters related to salt stress were measured and compared between transgenic and wild type (WT) plants. Our results indicate that overexpression of ThbZIP1 can enhance the activity of both peroxidase (POD) and superoxide dismutase (SOD), and increase the content of soluble sugars and soluble proteins under salt stress conditions. These results suggest that ThbZIP1 contributes to salt tolerance by mediating signaling through multiple physiological pathways. Furthermore, ThbZIP1 confers stress tolerance to plants by enhancing reactive oxygen species (ROS) scavenging, facilitating the accumulation of compatible osmolytes, and inducing and/or enhancing the biosynthesis of soluble proteins.
碱性亮氨酸拉链蛋白（bZIPs）是一类转录因子，能与脱落酸（ABA）响应元件（ABREs）结合，使植物能够耐受不利的环境条件。本研究从柽柳中克隆到一个新的 bZIP 基因 ThbZIP1。在柽柳中的表达研究表明，ThbZIP1 对 NaCl、聚乙二醇（PEG）6000、NaHCO3 和 CdCl2 的处理有差异调节，表明 ThbZIP1 参与非生物胁迫反应。为了鉴定 ThbZIP1 介导的生理反应，生成了过表达外源 ThbZIP1 的转基因烟草植物。测量了与盐胁迫相关的各种生理参数，并在转基因和野生型（WT）植物之间进行了比较。我们的结果表明，过表达 ThbZIP1 可以增强过氧化物酶（POD）和超氧化物歧化酶（SOD）的活性，并在盐胁迫条件下增加可溶性糖和可溶性蛋白的含量。这些结果表明，ThbZIP1 通过介导多条生理途径的信号转导，有助于盐胁迫耐受。此外，ThbZIP1 通过增强活性氧（ROS）清除、促进相容性渗透物的积累、诱导和/或增强可溶性蛋白的生物合成，赋予植物对胁迫的耐受性。
