Institute for Research in Biomedicine, Barcelona Science Park, Baldiri Reixac 10-12, E-08028 Barcelona, Spain.
Biol Chem. 2012 Sep 8;393(10):1165-71. doi: 10.1515/hsz-2012-0230.
The PhoR-PhoB phosphorelay is a bacterial two-component system that activates the transcription of several genes involved in phosphate uptake and assimilation. The response begins with the autophosphorylation of the sensor kinase PhoR, which activates the response regulator PhoB. Upon binding to the pho box DNA sequence, PhoB recruits the RNA polymerase and thereby activates the transcription of specific genes. To unveil hitherto unknown molecular mechanisms along the activation pathway, we report biochemical data characterizing the PhoB binding to promoters containing multiple pho boxes and describe the crystal structure of two PhoB DNA-binding domains bound in tandem to a 26-mer DNA.
PhoR-PhoB 磷酸传递系统是一种细菌双组分系统，可激活参与磷酸盐吸收和同化的几个基因的转录。该反应始于传感器激酶 PhoR 的自动磷酸化，该磷酸化激活了反应调节剂 PhoB。PhoB 与 pho 盒 DNA 序列结合后，招募 RNA 聚合酶，从而激活特定基因的转录。为了揭示激活途径中迄今未知的分子机制，我们报告了生化数据，这些数据描述了 PhoB 与包含多个 pho 盒的启动子结合的特征，并描述了两个串联结合到 26 个碱基对 DNA 的 PhoB DNA 结合域的晶体结构。
