Lu Minghua, Cai Zongwei
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, China.
Top Curr Chem. 2013;331:143-64. doi: 10.1007/128_2012_383.
Traditional Chinese medicines (TCMs) are attracting more and more attention because of their long historical clinical experience and reliable therapeutic efficacy for preventing and/or treating various human diseases. Many techniques and methods were developed for the analysis of TCMs to support new drug discovery and quality control. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), a soft ionization mass spectrometric technique, has been widely used in the analysis of a wide variety of large molecular compounds including proteins, peptides, and polymers since it was introduced in the late 1980s. In the present chapter, advances of MALDI-TOF MS in TCMs analysis have been reviewed. The review covers MALDI-TOF MS applications in the identification of new bioactive ingredients, analysis of alkaloids, determination of small molecular compounds with new matrices, proteomics analysis associated with TCMs, direct analysis of plant tissue, and other applications in TCMs.
中药因其悠久的临床历史经验以及在预防和/或治疗各种人类疾病方面可靠的治疗效果而受到越来越多的关注。人们开发了许多技术和方法用于中药分析，以支持新药发现和质量控制。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）是一种软电离质谱技术，自20世纪80年代末被引入以来，已广泛应用于分析包括蛋白质、肽和聚合物在内的各种大分子化合物。在本章中，对MALDI-TOF MS在中药分析方面的进展进行了综述。该综述涵盖了MALDI-TOF MS在鉴定新的生物活性成分、生物碱分析、用新基质测定小分子化合物、与中药相关的蛋白质组学分析、植物组织直接分析以及中药的其他应用等方面的应用。
