State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
Anal Chim Acta. 2010 Jan 4;657(1):9-18. doi: 10.1016/j.aca.2009.10.024. Epub 2009 Oct 20.
Traditional Chinese medicines (TCMs) are gaining more and more attention all over the world, due to their specific theory and long historical clinical practice. But the uncontrollable quality is a bottleneck for its modernization and globalization. This paper reviewed the recent analytical methods in the quality control of TCMs, including screening strategies of bioactive markers from TCMs through biochromatographic methods, the traditional chromatographic methods, DNA methods, as well as the spectroscopic methods, including FT-IR, NIR and NMR. The comprehensive methods, such as fingerprint and multi-component quantification are emphasized; hyphenated techniques, like HPLC-MS, GC-MS, CE-MS, LC-NMR, chemometric methods, and combination of chemical and biological methods, such as biofingerprint, metabolic fingerprint are now more and more widely used in TCMs. In a few word, the analysis and quality control of TCMs are moving towards an integrative and comprehensive direction, in order to better address the inherent holistic nature of TCMs.
中药（TCM）由于其独特的理论和长期的临床实践，在世界范围内受到越来越多的关注。但不可控的质量是其现代化和全球化的瓶颈。本文综述了中药质量控制的最新分析方法，包括通过生物色谱法筛选中药生物活性标志物的策略、传统色谱法、DNA 法以及包括傅里叶变换红外光谱（FT-IR）、近红外光谱（NIR）和核磁共振（NMR）在内的光谱法。强调了综合方法，如指纹图谱和多组分定量；现在越来越多的联用技术，如高效液相色谱-质谱联用（HPLC-MS）、气相色谱-质谱联用（GC-MS）、毛细管电泳-质谱联用（CE-MS）、液质联用（LC-NMR）、化学计量学方法以及化学和生物学方法的结合，如生物指纹图谱、代谢指纹图谱，越来越多地应用于中药。总之，中药的分析和质量控制正在朝着综合和全面的方向发展，以更好地解决中药固有的整体性。
