Faculty of Science, Department of Life Sciences M. Malpighi Section of General Physiology, University of Messina, Viale Ferdinando Stagno d'Alcontres, 31 S. Agata, 98166 Messina, Italy.
Cell Biochem Funct. 2013 Jul;31(5):412-6. doi: 10.1002/cbf.2915. Epub 2012 Oct 24.
The aim of the present study was to assess the effect of storage time at +4 °C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in mullet (Mugil cephalus) using an automatic method. After blood collection (T0 ), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t-test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4 °C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One-way repeated-measures ANOVA showed a significant effect of storage time (P < 0.05) on Hb, WBC, TC, MCH and MCHC. These results suggest that haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4 °C because long-term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers.
本研究旨在评估在 +4°C 下储存时间对鲻鱼（Mugil cephalus）的红细胞计数（RBC）、红细胞压积（Hct）、血红蛋白（Hb）、白细胞计数（WBC）、血小板计数（TC）、平均红细胞体积（MCV）、平均红细胞血红蛋白（MCH）和平均红细胞血红蛋白浓度（MCHC）的影响，采用自动方法。在采血后（T0），使用手动和自动方法对所有样本进行分析。为了测试自动方法的有效性，进行了配对 t 检验，未观察到统计学差异。将样本成功地分为四个不同的等分试样，并在 +4°C 下储存，使用自动方法评估血液学参数。第一个等分试样冷藏 6 小时，第二个等分试样冷藏 24 小时，第三个等分试样冷藏 48 小时，最后一个等分试样冷藏 72 小时。单向重复测量方差分析显示储存时间（P<0.05）对 Hb、WBC、TC、MCH 和 MCHC 有显著影响。这些结果表明，当样本在 +4°C 下储存时，从采血后 6 小时内可以评估血液学参数，因为长期储存会改变分析结果。在各种鱼类中，这些参数的进一步研究可能仍然需要，以验证一种有用的血液学分析方法，不仅可用于评估圈养和水产养殖动物的健康状况，还可作为可靠的环境血液学生物标志物。
