Mesalles Montse, Uroz Meritxell, Brandts Irene, Serrano Emmanuel, Cuenca Rafaela, Pastor Josep, Teles Mariana
Servei d'Hematologia Clínica Veterinària (SHCV), Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona (UAB), 08193 Cerdanyola del Vallès, Barcelona, Spain.
Departament de Biologia Celular, Fisiologia i Immunologia, Facultat de Biociencies, Universitat Autònoma de Barcelona (UAB), 08193 Cerdanyola del Vallès, Barcelona, Spain.
Animals (Basel). 2025 Apr 29;15(9):1265. doi: 10.3390/ani15091265.
Hematological studies provide essential information about the health of animals, which is crucial for veterinary medicine, scientific research, and aquaculture. Automatic hematological analyzers are an alternative to manual methods, offering faster and more reliable results. The objective of this study was to validate the Sysmex XN-1000V automatic hematology analyzer for blood samples from rainbow trout (), examine the effects of two anticoagulants (KEDTA and lithium heparin), and establish normal blood reference values for this fish species. Additionally, comparative studies were conducted between the Sysmex XN-1000V and manual methods (hemocytometer cell count and blood smear estimation), and reference intervals were established. Ninety-nine heparinized blood samples were analyzed for validation and sample stability tests. The results showed extremely good precision, with a coefficient of variation (CV) below 3% for RBCs, HGB, and HCT and less than 5% for non-RBC cells (leukocytes plus thrombocytes). However, heterophils (%) exhibited higher variability, with a CV of 15.08%. Linearity was excellent, and the carry-over was below 1% for all parameters. The sample stability test indicated that samples could be analyzed for up to 48 h when stored at 4 °C and up to 24 h at room temperature. Non-RBC cells were the first to degrade over time. The automated and manual methods demonstrated good correlation and agreement, validating the analyzer's accuracy. The effects of two anticoagulants, KEDTA and lithium heparin, on the blood samples were also studied. Heparin was the preferred anticoagulant for routine hematological analysis of rainbow trout blood with the Sysmex XN-1000V analyzer. In conclusion, the Sysmex XN-1000V enables complete hemogram analyses to be performed quickly and accurately, standardizing techniques, harmonizing results, and providing reliable reference intervals with blood.
血液学研究为动物健康提供了重要信息，这对兽医学、科学研究和水产养殖至关重要。自动血液分析仪是手工方法的替代选择，能提供更快且更可靠的结果。本研究的目的是验证Sysmex XN - 1000V自动血液分析仪对虹鳟鱼血液样本的适用性，研究两种抗凝剂（KEDTA和肝素锂）的影响，并建立该鱼类的正常血液参考值。此外，还在Sysmex XN - 1000V和手工方法（血细胞计数板细胞计数和血涂片评估）之间进行了比较研究，并确定了参考区间。对99份肝素化血液样本进行了分析，以进行验证和样本稳定性测试。结果显示精密度极佳，红细胞（RBC）、血红蛋白（HGB）和血细胞比容（HCT）的变异系数（CV）低于3%，非红细胞细胞（白细胞加血小板）的变异系数小于5%。然而，嗜中性粒细胞（%）的变异性较高，CV为15.08%。线性良好，所有参数的携带污染率均低于1%。样本稳定性测试表明，样本在4℃储存时可分析长达48小时，在室温下可分析长达24小时。非红细胞细胞随时间推移最先降解。自动化和手工方法显示出良好的相关性和一致性，验证了分析仪的准确性。还研究了两种抗凝剂KEDTA和肝素锂对血液样本的影响。肝素是使用Sysmex XN - 1000V分析仪对虹鳟鱼血液进行常规血液学分析的首选抗凝剂。总之，Sysmex XN - 1000V能够快速、准确地进行完整的血常规分析，规范技术，统一结果，并提供可靠的血液参考区间。
