Teagasc, Pig Development Department, Animal and Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork, Ireland.
J Anim Sci. 2013 Jan;91(1):318-30. doi: 10.2527/jas.2012-5360. Epub 2012 Oct 24.
This study assessed the effect of feeding genetically modified maize expressing a truncated form of the Cry1Ab protein from Bacillus thuringiensis (Bt MON810 maize) to sows during gestation and lactation and their offspring from weaning to 115 d postweaning on offspring growth and health. After weaning at approximately 28 d of age (d 0), individually penned, mixed sex pigs (approximately 8 kg BW) from sows fed isogenic or Bt maize diets were blocked by sow treatment, sex, and BW and randomly assigned to Bt or isogenic maize diets as follows: i) isogenic maize-fed sow/isogenic maize-fed offspring (iso/iso); ii) isogenic maize-fed sow/Bt maize-fed offspring (iso/Bt); iii) Bt maize-fed sow/isogenic maize-fed offspring (Bt/iso); and iv) Bt maize-fed sow/Bt maize-fed offspring (Bt/Bt). Growth performance was recorded at intervals to harvest at approximately 105 kg BW (n=15/treatment) and blood samples were taken for biochemical analysis on d 0, 30, 70, 100, and 115 postweaning (n=10/treatment). Pigs were harvested on d 115 postweaning (n=10/treatment), and carcass weight, backfat depth, and organ weights (heart, kidney, spleen, and liver) were recorded. Kidney, liver, lymph nodes, and small intestine were collected for histological analysis. Offspring from Bt maize-fed sows were heavier than offspring from isogenic maize-fed sows on d 30 (P<0.05), 100 (P<0.05), and 115 postweaning (P<0.05) and had greater overall ADG (P<0.05). Overall ADFI was greater for offspring from sows fed Bt maize (P<0.05) and for Bt maize-fed pigs (P<0.05). Offspring from Bt maize-fed sows had greater carcass (P<0.05) and lighter spleen (P<0.05) weights. Dressing percentage was greater for Bt maize-fed pigs than isogenic maize-fed pigs (P<0.05), and livers were lighter for pigs in the Bt/Bt group than pigs in the iso/Bt or Bt/iso group (P<0.05). Offspring from Bt maize-fed sows also had greater duodenal crypt depths (P<0.05) and lower villus height/crypt depth ratios (P<0.05). No pathology was observed in the organs, and serum biochemistry values generally remained within normal limits and no overall differences were observed, with the exception of overall γ glutamyltransferase, which was less for pigs on the Bt/Bt treatment than pigs on the iso/Bt and Bt/iso treatments. These results indicate that transgenerational consumption of Bt maize diets is not detrimental to pig growth and health.
本研究评估了在妊娠和哺乳期期间用表达苏云金芽孢杆菌 Cry1Ab 蛋白截短形式的转基因玉米（Bt MON810 玉米）喂养母猪及其后代从断奶到断奶后 115 天对后代生长和健康的影响。大约在 28 天大断奶时（d 0），从喂食同基因或 Bt 玉米饮食的母猪中取出单独圈养的、混合性别的猪（约 8kgBW），按母猪处理、性别和 BW 分组，并随机分配到 Bt 或同基因玉米饮食中，如下所示：i）同基因玉米喂养的母猪/同基因玉米喂养的后代（iso/iso）；ii）同基因玉米喂养的母猪/Bt 玉米喂养的后代（iso/Bt）；iii）Bt 玉米喂养的母猪/同基因玉米喂养的后代（Bt/iso）；iv）Bt 玉米喂养的母猪/Bt 玉米喂养的后代（Bt/Bt）。在大约 105kgBW 时进行间隔生长性能记录（n=15/处理），并在断奶后第 0、30、70、100 和 115 天（n=10/处理）采集血液样本进行生化分析。在断奶后第 115 天（n=10/处理）进行猪的屠宰，记录胴体重、背膘厚度和器官重量（心脏、肾脏、脾脏和肝脏）。收集肾脏、肝脏、淋巴结和小肠进行组织学分析。与同基因玉米喂养的母猪的后代相比，Bt 玉米喂养的母猪的后代在断奶后第 30、100 和 115 天（P<0.05）体重更大，总 ADG 更高（P<0.05）。Bt 玉米喂养的后代的总 ADFI 更高（P<0.05）。与同基因玉米喂养的母猪的后代相比，Bt 玉米喂养的母猪的后代的胴体（P<0.05）和脾脏（P<0.05）更轻。与同基因玉米喂养的猪相比，Bt 玉米喂养的猪的屠宰率更高（P<0.05），Bt/Bt 组的猪的肝脏比 iso/Bt 或 Bt/iso 组的猪更轻（P<0.05）。Bt 玉米喂养的母猪的后代的十二指肠隐窝深度也更大（P<0.05），绒毛高度/隐窝深度比值更低（P<0.05）。各器官未观察到病理学变化，血清生化值通常保持在正常范围内，未观察到总体差异，除了总γ谷氨酰转移酶外，Bt/Bt 处理的猪的该值低于 iso/Bt 和 Bt/iso 处理的猪。这些结果表明，转基因玉米饲料的跨代消耗对猪的生长和健康没有不利影响。
