从产几丁质酶和抗菜豆根腐病原菌活性发生改变的粘质沙雷氏菌 PM-4 中分离和鉴定突变体。
Isolation and characterization of mutants of Pseudomonas maltophila PM-4 altered in chitinolytic activity and antagonistic activity against root rot pathogens of clusterbean (Cyamopsis tetragonoloba).
机构信息
Department of Microbiology, CCS Haryana Agricultural University, Hisar, 125 004 India.
出版信息
Indian J Microbiol. 2007 Mar;47(1):64-71. doi: 10.1007/s12088-007-0012-z. Epub 2007 Jun 14.
Pseudomonas maltophila PM-4, an antagonist of pathogenic fungi including Rhizoctonia bataticola, R. solani, Fusarium oxysporum and Sclerotinia sclerotiorum associated with root rot of clusterbean (Cyamopsis tetragonoloba) was mutagenized with Tn5. Hyperchitinase producing mutants showing large zone of colloidal chitin dissolution were identified on medium containing calcoflor dye as an indicator. A mutant P-48 producing 137% higher chitinase activity than the parent strain PM-4 was identified. Seed bacterization of clusterbean (Cyamopsis tetragonoloba) with P-48 controlled the root rot upto 40.8% in the presence of conglomerate of all the four fungal pathogens Rhizoctonia bataticola, R. solani, F. oxysporum and Sclerotinia sclerotiorum.
假单胞菌 PM-4 是一种拮抗真菌的物质，包括与豇豆根腐病相关的炭疽病菌、丝核菌、尖孢镰刀菌和核盘菌。该菌使用 Tn5 进行了诱变。在含有 Calcofluor 染料作为指示剂的培养基上，发现了产生大量胶体几丁质溶解区的高几丁质酶产生突变体。鉴定出一个突变体 P-48 的几丁质酶活性比亲本菌株 PM-4 高 137%。用 P-48 对豇豆（菜豆）进行种子细菌接种，在四种真菌病原体炭疽病菌、丝核菌、尖孢镰刀菌和核盘菌的混合体存在的情况下，可控制根腐病，发病率降低 40.8%。
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