State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, P.R. China.
Department of Plant Pathology, Washington State University, Pullman 99164-6430, U.S.A.
Phytopathology. 2020 May;110(5):1010-1017. doi: 10.1094/PHYTO-09-19-0367-R. Epub 2020 Mar 24.
A four-gene operon () from Pf-5 encoding the biosynthesis of the antibiotic pyrronitrin was introduced into (formerly ) 2-79, an aggressive root colonizer of both dryland and irrigated wheat roots that naturally produces the antibiotic phenazine-1-carboxylic acid and suppresses both take-all and Rhizoctonia root rot of wheat. Recombinant strains ZHW15 and ZHW25 produced both antibiotics and maintained population sizes in the rhizosphere of wheat that were comparable to those of strain 2-79. The recombinant strains inhibited in vitro the wheat pathogens anastomosis group 8 (AG-8) and AG-2-1, var. , , , and significantly more than did strain 2-79. Both the wild-type and recombinant strains were equally inhibitory of . When applied as a seed treatment, the recombinant strains suppressed take-all, Rhizoctonia root rot of wheat, and Rhizoctonia root and stem rot of canola significantly better than did wild-type strain 2-79.
一个来自 Pf-5 的四基因操纵子（），其编码抗生素吡咯并菌素的生物合成，被引入到（以前称为）2-79 中，这是一种干旱和灌溉小麦根系的侵略性根定殖者，它自然产生抗生素吩嗪-1-羧酸，并抑制全蚀病和小麦纹枯病。重组菌株 ZHW15 和 ZHW25 同时产生这两种抗生素，并在小麦根际保持与菌株 2-79 相当的种群数量。重组菌株在体外显著抑制小麦病原菌 8 号（AG-8）和 AG-2-1、变种、、、和 ，比菌株 2-79 更为显著。野生型和重组菌株对 同样具有抑制作用。当作为种子处理剂应用时，重组菌株对小麦全蚀病、纹枯病和油菜根和茎腐病的抑制作用明显优于野生型菌株 2-79。
