松针单宁提取物对一些具有农业利用价值的微生物的抑制活性。
Inhibitory activity of pine needle tannin extracts on some agriculturally resourceful microbes.
机构信息
Vivekananda Parvatiya Krishi Anusandhan Sansthan (ICAR), Almora, 263 601 Uttarakhand India.
出版信息
Indian J Microbiol. 2007 Sep;47(3):267-70. doi: 10.1007/s12088-007-0049-z. Epub 2007 Oct 4.
Crude extracts of water and solvent extractable tannin fractions from pine needles were found to contain tannin concentrations of 10.15% and 13.15% tannic acid equivalents respectively. Thin Layer Chromatography revealed the presence of four distinct phenolic compounds, amongst which two were tannic acid like compounds. Both the extracts were found to be inhibitory to several microbes of agricultural importance. Amongst the bacterial strains studied, Azotobacter sp (VL-A2) was able to tolerate upto 1000 ppm of crude tannin concentration without any growth inhibition. While growth of Rhizobium (VL-R1) and Bacillus halodurans (MTCC 7181) was inhibited by crude tannin concentrations of 50 and 100 ppm respectively of both water and solvent extracted tannins. Among the fungal genera, Pleurotus djamor was found to tolerate up to 10000 ppm of crude tannins, while Trichoderma virescens (MTCC 6321) and T. reesii could tolerate up to 3000 ppm of both water extractable and acetone extractable crude tannins without any growth inhibition.
松针的水提和溶剂可提单宁提取物粗提物中分别含有 10.15%和 13.15%的单宁酸当量。薄层层析显示存在四种不同的酚类化合物，其中两种类似于单宁酸的化合物。这两种提取物都对几种具有农业重要性的微生物具有抑制作用。在所研究的细菌菌株中，固氮菌（VL-A2）能够耐受高达 1000ppm 的粗单宁浓度而没有任何生长抑制。而根瘤菌（VL-R1）和芽孢杆菌（MTCC 7181）的生长分别被水提和溶剂提单宁的 50ppm 和 100ppm 的粗单宁浓度所抑制。在真菌属中，发现平菇可以耐受高达 10000ppm 的粗单宁，而木霉（MTCC 6321）和瑞氏木霉（T. reesii）可以耐受高达 3000ppm 的水提和丙酮提粗单宁而没有任何生长抑制。
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