用γ射线照射制备口蹄疫 A 型 A87/IRN 灭活疫苗及豚鼠免疫应答的研究。
Preparation of FMD type A87/IRN inactivated vaccine by gamma irradiation and the immune response on guinea pig.
机构信息
Agricultural and Industrial Research school-Nuclear science and technology Research Institute of Iran, Karaj, Iran.
出版信息
Indian J Microbiol. 2008 Sep;48(3):326-30. doi: 10.1007/s12088-008-0023-4. Epub 2008 Jun 13.
FMD is one of the most economically damaging diseases that affect livestock animals. In this study FMD Virus type A87/IRN was multiplied on BHK21 cells. The virus was titrated by TCID50 method, it was 10(7.5)/ml. The FMD virus samples were inactivated by gamma ray from 60Co source at -20°C. Safety test was done by IBRS2 monolayer cell culture method, also antigenicity of irradiated and un-irradiated virus samples were studied by Complement Fixation Test. The Dose/Survival curve for irradiated FMD Virus was drawn, the optimum dose range for inactivation of FMDV type A87/IRN and unaltered antigenicity was obtained 40-44 kGy. The inactivated virus samples by irradiation and ethyleneimine (EI) were formulated respectively as vaccine with Al(OH)3 gel and other substances. The vaccines were inoculated to Guinea pigs and the results of Serum Neutralization Test for the normal vaccine and radio-vaccine showed protective titer after 8 months. The potency test of the inactivated vaccines was done, PD50 Value of the vaccines were calculated 7.06 and 5.6 for inactivated vaccine by EI and gamma irradiation respectively.
口蹄疫是一种对家畜危害极大的疾病。本研究在 BHK21 细胞上增殖了口蹄疫病毒 A 型 87/IRN。采用 TCID50 法滴定病毒滴度，为 10(7.5)/ml。用 60Co 源γ射线在-20°C 下使口蹄疫病毒样品失活。采用 IBRS2 单层细胞培养法进行安全性试验，并用补体结合试验研究了辐照和未辐照病毒样品的抗原性。绘制了辐照口蹄疫病毒的剂量/存活率曲线，得出了使 A 型 87/IRN 口蹄疫病毒失活且不改变抗原性的最佳剂量范围为 40-44 kGy。分别用辐照和乙撑亚胺（EI）处理病毒样品，并用 Al(OH)3 凝胶和其他物质配制疫苗。将疫苗接种给豚鼠，正常疫苗和放射疫苗的血清中和试验结果显示，接种 8 个月后具有保护效价。对灭活疫苗进行效力试验，用 EI 和γ射线处理的灭活疫苗的 PD50 值分别为 7.06 和 5.6。
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