Donn A, Castagnaro M, Donaldson A I
Institute for Animal Health, Pirbright Laboratory, Woking, U.K.
Arch Virol. 1995;140(1):13-25. doi: 10.1007/BF01309720.
Persistent foot-and-mouth disease (FMD) virus infection in vitro has been studied in a chronically infected cloned BHK-21 cell line. Virus growth during serial cell passages was followed by infectivity assay and immunocytochemical staining. Only a small percentage of cells (0.006-6%) was found to harbour virus during persistence. Light and electron microscopy showed the presence of cytoplasmic protuberances ("blebs") at the surface of persistently infected cells. The curing of cell cultures was achieved by passaging them in the presence of polyvalent immune serum. The absence of virus in cured cells was confirmed by infectivity assay and immunocytochemistry. This finding, together with the low percentage of infected cells in cultures confirms that persistently infected BHK-21 cells satisfy the definitions of a carrier culture. The characteristics of the in vitro system and its relevance to the study of FMD carrier state in vivo are discussed.
在一个慢性感染的克隆BHK - 21细胞系中，对口蹄疫（FMD）病毒的持续性体外感染进行了研究。通过感染性测定和免疫细胞化学染色追踪了病毒在连续细胞传代过程中的生长情况。在持续性感染期间，仅发现一小部分细胞（0.006 - 6%）携带病毒。光学显微镜和电子显微镜显示，持续性感染细胞表面存在细胞质突起（“泡”）。通过在多价免疫血清存在的情况下传代细胞培养物，实现了细胞培养物的治愈。通过感染性测定和免疫细胞化学证实了治愈细胞中不存在病毒。这一发现，连同培养物中感染细胞的低比例，证实了持续性感染的BHK - 21细胞符合载体培养物的定义。讨论了体外系统的特征及其与体内口蹄疫载体状态研究的相关性。
