白色链霉菌产 L-天冬酰胺酶。
L-asparaginase production by Streptomyces albidoflavus.
机构信息
Acharya Nagarjuna University, Guntur, 522 510, AP India.
出版信息
Indian J Microbiol. 2008 Sep;48(3):331-6. doi: 10.1007/s12088-008-0018-1. Epub 2008 Jun 12.
Abstract
Attempts were made to optimize the cultural conditions for the production of L-asparaginase by Streptomyces albidoflavus under submerged fermentations. Enhanced level of L-asparaginase was found in culture medium supplemented with maltose as carbon source. Yeast extract (2%) was served as good nitrogen source for the production of L-asparaginase. The optimum pH for enzyme production was 7.5 and temperature was 35°C. The release of L-asparaginase from the cells of S. albidoflavus was high when strain was treated with cell disrupting agents like EDTA and lysozyme. The enzyme produced by the strain was purifi ed by ammonium sulfate, Sephadex G-100 and CM-Sephadex C-50 gel fi ltration and the molecular weight was apparently determined as 112 kDa.
摘要
尝试通过深层发酵优化白色链霉菌生产 L-天冬酰胺酶的培养条件。在添加麦芽糖作为碳源的培养基中发现 L-天冬酰胺酶的含量有所提高。酵母提取物（2%）是生产 L-天冬酰胺酶的良好氮源。产酶的最适 pH 值为 7.5，温度为 35°C。当用 EDTA 和溶菌酶等细胞裂解剂处理白色链霉菌的细胞时，L-天冬酰胺酶从细胞中的释放量很高。该菌株产生的酶通过硫酸铵、葡聚糖凝胶 G-100 和 CM-葡聚糖凝胶 C-50 凝胶过滤进行纯化，分子量明显确定为 112 kDa。
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