为仿生二氧化碳捕集而对不同属的碳酸酐酶进行的特性描述。
Characterization of carbonic anhydrase from diversified genus for biomimetic carbon-dioxide sequestration.
机构信息
Bacteriology Laboratory, Department of P.G studies and Research in Biological Sciences, R.D University, Jabalpur, 482 001 (M.P.) India.
出版信息
Indian J Microbiol. 2008 Sep;48(3):365-71. doi: 10.1007/s12088-008-0045-y. Epub 2009 Mar 25.
Diversified group of bacteria were screened for carbonic anhydrase (CA) activity. Significant CA activity was found in crude enzyme extracts of Enterobacter and Aeromonas isolates while minimal or negligible CA activity was observed in case of Shigella and Klebsiella spp. Optimization and characterization study of potent CA producing isolates revealed that the maximum enzyme activity of 3.86 EU/ml was observed in E. taylorae and the optimum pH range for enzyme stability was found to be 7.5-9.0 along with an optimum temperature range of 35-50 °C. The molecular mass of CA was 29-kDa indicating α-type with periplasmic and cytosolic location. Present investigation for the first time reports CA in diversified genus and optimized parameters for enhanced production of CA in Enterobacter sp. & Aeromonas sp. from fresh water bodies that inturn lay down grounds for exploitation of CA from E. taylorae as an efficient catalyst for CO(2) sequestration within a bioreactor.
对多种细菌进行了碳酸酐酶 (CA) 活性筛选。在肠杆菌和气单胞菌分离株的粗酶提取物中发现了显著的 CA 活性，而在志贺氏菌和克雷伯氏菌属中则观察到最小或可忽略不计的 CA 活性。对产 CA 能力强的分离株的优化和特性研究表明，在 E. taylorae 中观察到最大酶活为 3.86 EU/ml，并且发现酶稳定性的最佳 pH 范围为 7.5-9.0，最佳温度范围为 35-50°C。CA 的分子量为 29-kDa，表明为 α 型，具有周质和细胞质定位。本研究首次报道了在多种属中存在 CA，并优化了从淡水体中增强肠杆菌属和气单胞菌属 CA 生产的参数，这为从 E. taylorae 中开发 CA 作为生物反应器中 CO(2) 固定的有效催化剂奠定了基础。
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