检测从鱼类和池塘水中分离的嗜水气单胞菌中的 aerolysin 基因。
Detection of aerolysin gene in Aeromonas hydrophila isolated from fish and pond water.
机构信息
Aquatic Microbes Section, National Bureau of Fish Genetic Resources, Canal Ring Road, PO Dilkusha, Lucknow, 226 002 India.
出版信息
Indian J Microbiol. 2008 Dec;48(4):453-8. doi: 10.1007/s12088-008-0056-8. Epub 2009 Jan 8.
Abstract
Aerolysin is a hemolytic toxin encoded by aerolysin gene (1482 bp) that plays a key role in the pathogenesis of Aeromonas hydrophila infection in fish. New speciesspecific primers were designed to amplify 326 bp conserved region of aerolysin gene for A. hydrophila. Twenty-five isolates of A. hydrophila recovered from fish and pond water were studied for detection of aerolysin gene. Aerolysin gene was detected in 85% of the isolates during the study. The designed primers were highly specific and showed no cross reactivity with Escherichia coli, Aeromonas veronii, Vibrio cholerae, Flavobacterium spp., Chyseobacterium spp. and Staphylococcus aureus. The sensitivity limit of primers for detection of aerolysin gene in the genomic DNA of A. hydrophila was 5 pg.
摘要
气溶素是一种溶血毒素，由气溶素基因（1482bp）编码，在鱼类致病性嗜水气单胞菌感染中发挥关键作用。设计了新的种特异性引物来扩增气溶素基因的 326bp 保守区，用于检测嗜水气单胞菌。从鱼类和池塘水中分离出的 25 株嗜水气单胞菌进行了气溶素基因检测。在研究期间，85%的分离株检测到气溶素基因。设计的引物具有高度特异性，与大肠杆菌、维氏气单胞菌、霍乱弧菌、黄杆菌属、希瓦氏菌属和金黄色葡萄球菌无交叉反应。引物用于检测嗜水气单胞菌基因组 DNA 中气溶素基因的灵敏度限为 5pg。
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本文引用的文献
1
Detection and characterization of the hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR.用多重聚合酶链反应检测和鉴定嗜水气单胞菌和温和气单胞菌中的溶血素基因
J Clin Microbiol. 2003 Mar;41(3):1048-54. doi: 10.1128/JCM.41.3.1048-1054.2003.
2
Secreted enzymes of Aeromonas.气单胞菌的分泌酶
FEMS Microbiol Lett. 1997 Jul 1;152(1):1-10. doi: 10.1111/j.1574-6968.1997.tb10401.x.
3
Identification of Aeromonas hydrophila hybridization group 1 by PCR assays.通过聚合酶链反应（PCR）检测鉴定嗜水气单胞菌杂交组1
Appl Environ Microbiol. 1996 Apr;62(4):1167-70. doi: 10.1128/aem.62.4.1167-1170.1996.
4
Emerging pathogens: Aeromonas spp.新出现的病原体：气单胞菌属
Int J Food Microbiol. 1995 Dec;28(2):157-68. doi: 10.1016/0168-1605(95)00054-2.
5
Cloning, expression, and sequence analysis of a cytolytic enterotoxin gene from Aeromonas hydrophila.嗜水气单胞菌细胞溶解性肠毒素基因的克隆、表达及序列分析
Can J Microbiol. 1993 May;39(5):513-23. doi: 10.1139/m93-073.
6
Detection of aerolysin gene in Aeromonas strains isolated from drinking water, fish and foods by the polymerase chain reaction.通过聚合酶链反应检测从饮用水、鱼类和食品中分离出的气单胞菌菌株中的气溶素基因。
Comp Immunol Microbiol Infect Dis. 1995 Jan;18(1):17-26. doi: 10.1016/0147-9571(94)e0001-a.
7
Structure of the Aeromonas toxin proaerolysin in its water-soluble and membrane-channel states.气单胞菌毒素前气溶素在其水溶性和膜通道状态下的结构。
Nature. 1994 Jan 20;367(6460):292-5. doi: 10.1038/367292a0.
8
Activation of the hole-forming toxin aerolysin by extracellular processing.通过细胞外加工激活成孔毒素气单胞菌溶素。
J Bacteriol. 1985 Jul;163(1):336-40. doi: 10.1128/jb.163.1.336-340.1985.
9
Nucleotide sequence of the gene for the hole-forming toxin aerolysin of Aeromonas hydrophila.嗜水气单胞菌成孔毒素气溶素基因的核苷酸序列。
J Bacteriol. 1987 Jun;169(6):2869-71. doi: 10.1128/jb.169.6.2869-2871.1987.
10
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J Clin Microbiol. 1990 Nov;28(11):2477-81. doi: 10.1128/jcm.28.11.2477-2481.1990.
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