Centre for Post Harvest Engineering and Technology, Ludhiana, India.
Indian J Microbiol. 2010 Mar;50(1):12-8. doi: 10.1007/s12088-009-0018-9. Epub 2009 Mar 27.
The potential of Nostoc calcicola and its bicarbonate resistant mutant as bioameleorating agent was investigated, using laboratory simulation experiments, in terms of their growth potential, glutamine synthetase (GS) activity, heterocyst frequency and effect on pH of soil. Nostoc calcicola, exhibited a tendency to lower the pH of 'usar' soil significantly and showed better growth and pigment content at 20% soil extract as compared to basal medium. The bicarbonate resistant mutant (HCO(3) (-R)) exhibited a better ability to grow at higher percentage of soil extract (60%), besides bringing about a more significant change in soil pH as compared to wild type. The heterocyst frequency was much higher in the mutant strain, which was not significantly affected by growth in various concentrations of soil extract. The mutant strain holds promise as a potential bioameliorant for 'usar' soil after further evaluation of its reclamative properties at field level.
研究了念珠藻及其耐碳酸氢盐突变体作为生物改良剂的潜力，通过实验室模拟实验，从生长潜力、谷氨酰胺合成酶（GS）活性、异形胞频率以及对土壤 pH 的影响等方面进行了研究。与基础培养基相比，念珠藻表现出显著降低“usah”土壤 pH 的趋势，在 20%土壤提取物中的生长和色素含量更好。耐碳酸氢盐突变体（HCO(3) (-R)）在更高比例的土壤提取物（60%）中表现出更好的生长能力，与野生型相比，土壤 pH 的变化更为显著。异形胞频率在突变株中更高，而在不同浓度土壤提取物中的生长对其影响不大。在进一步评估其在田间的修复特性后，该突变株有望成为“usah”土壤的潜在生物改良剂。
