Department of Biochemistry, Shivaji University, Kolhapur, 416004 India ; National University of Ireland Galway, University Road Galway, Galway, Ireland.
Indian J Microbiol. 2010 Jun;50(2):156-64. doi: 10.1007/s12088-010-0001-5. Epub 2010 Mar 5.
A high exhaust reactive dye, Green HE4B (GHE4B) was 98% degraded in nutrient medium by Pseudomonas desmolyticum NCIM 2112 (pd2112) within 72 h at static condition. Decolorization time in synthetic 10 g/l molasses. Addition of 5 g/l peptone to NaCl medium had reduced decolorization time from 108 to 72 h. Beef extract do not contribute more to the inducing effect of peptone, however it is a good co-substrate in sucrose or urea containing NaCl medium. Intracellular lignin peroxidase (Lip), laccase and tyrosinase activities were induced by 150, 355 and 212%, respectively till maximum dye removal took place. Aminopyrine N-demethylase (AND) and dichlorophenol indophenol reductase (DCIP-reductase) activities in pd2112 were induced by 130 and 20%, respectively at 72 h of incubation during GHE4B decolorization. By high performance liquid chromatography (HPLC) analysis, 4-hydroxybenzene sulfonic acid and 4-amino, 6-hydroxynaphthalene 2-sulfonic acids were identified as metabolites formed during 24-72 h incubation. Fourier transform infrared spectroscopy (FTIR) analysis supports the formation of these aromatic amines. pd2112, aerobically degraded GHE4B metabolites (formed at static condition) showing stationary phase of 6 days. There was no germination inhibition of Sorghum bicolor and Triticum aestivum by GHE4B metabolites at 3,000 ppm concentration however untreated dye showed germination inhibition at the same concentration. GHE4B metabolites did not show any microbial toxicity at 10,000 ppm concentration.
一株高效好氧脱氮假单胞菌（Pseudomonas desmolyticum NCIM 2112，pd2112）在静置条件下，72 小时内可将高比活度的绿色 HE4B 染料（GHE4B）降解 98%。在 10g/L 糖蜜的合成培养基中，脱色时间为 72 小时。在含 NaCl 的培养基中添加 5g/L 蛋白胨可将脱色时间从 108 小时缩短至 72 小时。牛肉膏对蛋白胨的诱导作用没有贡献，但在含有蔗糖或尿素的 NaCl 培养基中是一种良好的共底物。胞内木质素过氧化物酶（Lip）、漆酶和酪氨酸酶的活性分别诱导至 150%、355%和 212%，直至最大染料去除率达到最大值。在 GHE4B 脱色过程中，72 小时时 Aminopyrine N-demethylase（AND）和 Dichlorophenol indophenol reductase（DCIP-reductase）的活性分别诱导至 130%和 20%。通过高效液相色谱（HPLC）分析，在 24-72 小时孵育期间，鉴定出 4-羟基苯磺酸和 4-氨基、6-羟基萘 2-磺酸为染料降解过程中形成的代谢物。傅里叶变换红外光谱（FTIR）分析支持这些芳香胺的形成。pd2112 有氧降解 GHE4B 代谢物（在静置条件下形成）显示出 6 天的稳定期。在 3000ppm 浓度下，GHE4B 代谢物对 Sorghum bicolor 和 Triticum aestivum 没有发芽抑制作用，而未经处理的染料在相同浓度下表现出发芽抑制作用。在 10000ppm 浓度下，GHE4B 代谢物没有显示出任何微生物毒性。
