Kalme Satish, Parshetti Ganesh, Gomare Sushma, Govindwar Sanjay
Department of Biochemistry, Shivaji University, Kolhapur, 416004, India.
Curr Microbiol. 2008 Jun;56(6):581-6. doi: 10.1007/s00284-008-9128-6. Epub 2008 Mar 11.
Pseudomonas desmolyticum NCIM 2112 (Pd 2112) and Nocardia hydrocarbonoxydans NCIM 2386 (Nh 2386) demonstrated an ability to degrade diesel and kerosene. Triton X-100 had enhanced the diesel degradation process by reducing the time required for the maximum utilization of total petroleum hydrocarbon. Fourier transform infrared spectroscopy spectrum of degraded diesel indicates the presence of aliphatic and aromatic aldehydes, C=C aromatic nuclei, and substituted benzenes. Surface tension reduction and stable emulsification was increased using consortium when compared to individual strains. Triton X-100 showed increase in microbial attachment to hydrocarbon among the various chemical surfactants tested. For generating a rapid assay to screen microorganisms capable of degrading kerosene, the acetaldehyde produced in the degradation process could be used as an indicator of degradation. These results indicate diesel and kerosene degradation ability of both of the strains.
解脂假单胞菌NCIM 2112（Pd 2112）和氧化烃诺卡氏菌NCIM 2386（Nh 2386）表现出降解柴油和煤油的能力。吐温X-100通过减少总石油烃最大利用所需的时间，增强了柴油降解过程。降解柴油的傅里叶变换红外光谱表明存在脂肪族和芳香族醛、C=C芳香核以及取代苯。与单个菌株相比，使用混合菌群时表面张力降低和稳定乳化作用增强。在测试的各种化学表面活性剂中，吐温X-100显示微生物对烃的附着增加。为了建立一种快速检测方法来筛选能够降解煤油的微生物，降解过程中产生的乙醛可作为降解的指标。这些结果表明这两种菌株都具有柴油和煤油降解能力。
