National Bureau of Agriculturally Important Microorganisms (NBAIM), Mau, Uttar Pradesh, India.
Indian J Microbiol. 2010 Jun;50(2):199-204. doi: 10.1007/s12088-010-0033-x. Epub 2010 Mar 16.
Genetic diversity analysis of Macrophomina phaseolina isolates obtained from different host range and diverse geographical locations in India was carried out using RAPD fingerprinting. Of the thirteen 10-mer random primers used, primer OPB-08 gave the maximum polymorphism and the UPGMA clustering could separate 50 isolates in to ten groups at more than 65% similarity level. The ten clusters correlated well with the geographical locations with exceptions for isolates obtained from Eastern and Western Ghats. There was a segregation of isolates from these two geographical locations in to two clusters thus, distributing 10 genotypes in to eight geographical locations. All the isolates M. phaseolina irrespective of their host and geographical origin, exhibited two representative monomorphic bands at 250 bp and 1 kb, presence of these bands suggests that isolates might have evolved from a common ancestor but due to geographical isolation fallowed by natural selection and genetic drift might have segregated in to subpopulations. Genetic similarity in the pathogenic population reflects the dispersal of single lineage in all locations in India.
利用 RAPD 指纹技术对从印度不同宿主范围和不同地理位置获得的拟茎点霉分离物进行遗传多样性分析。在使用的 13 个 10 碱基随机引物中，引物 OPB-08 产生最大的多态性，UPGMA 聚类可将 50 个分离物在相似度超过 65%的水平上分为 10 组。这十个聚类与地理位置很好地相关，除了从东高止山脉和西高止山脉获得的分离物。这两个地理位置的分离物分离成两个聚类，因此，将 10 种基因型分布在 8 个地理位置。所有的拟茎点霉分离物，无论其宿主和地理位置如何，都表现出两条代表单态性的条带，位于 250bp 和 1kb 处，这些条带的存在表明分离物可能是从一个共同的祖先进化而来的，但由于地理隔离，随后的自然选择和遗传漂变可能使它们分离成亚种群。致病种群的遗传相似性反映了单一线系在印度所有地点的传播。
