高锰酸钾-乙二胺四乙酸协同作用对人A、B型红细胞凝集性的强效抑制
Potent suppression of agglutinability of human A and B type erythrocytes by KMnO(4)-EDTA concerted action.
作者信息
Ghosh Dipankar, Santra Susmita, Dutta Sujit, Pramanik Panchanan
机构信息
Department of Chemistry, Indian Institute of Technology, Kharagpur, 721 302 India.
出版信息
Indian J Hematol Blood Transfus. 2009 Sep;25(3):104-10. doi: 10.1007/s12288-009-0022-6. Epub 2009 Nov 12.
PURPOSE OF THE STUDY
Study was focused to effectively suppress the agglutinability of human A and B type erythrocyte by concerted chemical oxidation approach. Consequently some major bio-physiological consequences of human erythrocytes were analyzed.
MATERIALS AND METHODS
Concerted chemical oxidation of human A and B type erythrocyte were done by potassium permanganate (KMnO(4)) in presence of ethylene diamine tetra-acetic acid (EDTA). All the chemical reactions were carried out at 37°C temperature and pH 7.4 in phosphate buffer. Finally, suppression of agglutinability were determined by using anti-A and anti-B antisera.
RESULTS
A marked suppression in agglutinability was observed after chemical treatment on human A and B type erythrocytes in vitro. Effective suppression of agglutinability was observed in 0.57 mM concentration of KMnO(4) in presence of 0.9 mM EDTA at optimum condition.
CONCLUSIONS
Results showed that KMnO(4)-EDTA concerted action is feasible. The future work is directed towards the universal human erythrocytes preparation from A and B type human erythrocytes.
研究目的
本研究聚焦于通过协同化学氧化方法有效抑制人A、B型红细胞的凝集性。因此，对人红细胞的一些主要生物生理后果进行了分析。
材料与方法
在乙二胺四乙酸（EDTA）存在的情况下，用高锰酸钾（KMnO₄）对人A、B型红细胞进行协同化学氧化。所有化学反应均在37℃温度和pH 7.4的磷酸盐缓冲液中进行。最后，使用抗A和抗B抗血清测定凝集性的抑制情况。
结果
体外对人A、B型红细胞进行化学处理后，观察到凝集性有显著抑制。在最佳条件下，在0.9 mM EDTA存在的情况下，0.57 mM浓度的KMnO₄可有效抑制凝集性。
结论
结果表明KMnO₄ - EDTA协同作用是可行的。未来的工作旨在从人A、B型红细胞制备通用型人红细胞。
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