共轭聚合物在杂化纳米球上的自组装及其在细胞成像中的应用。

Self-assembly of conjugated polymer on hybrid nanospheres for cellular imaging applications.

机构信息

State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China.

出版信息

ACS Appl Mater Interfaces. 2012 Nov;4(11):6332-7. doi: 10.1021/am301945k. Epub 2012 Nov 2.

DOI:10.1021/am301945k

PMID:23101490

Abstract

A new kind of hybrid core-shell nanosphere was fabricated by combining the in situ formation of Au nanoparticles and covalent cross-linking of biocompatible carboxymethyl starch dialdehyde (CMSD) and chitosan (CTS). When the fluorescent dye poly[9,9'-bis(6″-(N,N,N-trimethylammonium)-hexyl)fluorene-2,7-ylenevinylene-co-alt-1,4-phenylene dibromide] (PFV) was assembled on the surface of the hybrid nanospheres through electrostatic attraction, these biocompatible hybrid nanospheres exhibited metal-enhanced fluorescence effects. The fluorescence intensity of (CTS-Au)@CMSD/PFV hybrid nanosphere is 1.43 times that of CTS-CMSD/PFV hybrid nanospheres lacking Au nanoparticle. In addition, the (CTS-Au)@CMSD/PFV hybrid nanospheres exhibit excellent biodegradability upon exposure to enzymatic aqueous solution and good biocompatibility when cocultured with HeLa cervical carcinoma cells; these advantages make them attractive for cellular imaging and biological analysis and detection.

摘要

通过将金纳米粒子的原位形成与生物相容性的羧甲基淀粉二醛（CMSD）和壳聚糖（CTS）的共价交联相结合，制备了一种新型的杂化核壳纳米球。当荧光染料聚[9,9'-双（6"-（N，N，N-三甲基铵）-己基）芴-2,7-亚乙烯基-co-alt-1,4-亚苯基二溴]（PFV）通过静电吸引组装在杂化纳米球的表面上时，这些生物相容性的杂化纳米球表现出金属增强的荧光效应。（CTS-Au）@CMSD/PFV 杂化纳米球的荧光强度是缺乏金纳米粒子的 CTS-CMSD/PFV 杂化纳米球的 1.43 倍。此外，（CTS-Au）@CMSD/PFV 杂化纳米球在暴露于酶水溶液时表现出优异的可生物降解性，并且与 HeLa 宫颈癌细胞共培养时具有良好的生物相容性；这些优点使它们在细胞成像和生物分析及检测方面具有吸引力。