School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China.
Langmuir. 2010 Jul 20;26(14):11774-8. doi: 10.1021/la101714q.
A novel fluorescent nanoparticle was prepared via a simple self-assembly technique based on water-soluble conjugated polymers (CPs) and Ag@SiO(2) core-shell nanoparticles. Core-shell nanoparticles with silver NPs core show a unique property referred to as metal-enhanced fluorescence (MEF). In the present work, the cationic conjugated polymer poly[9,9'-bis(6''-(N,N,N-trimethylammonium)-hexyl) fluorene-2,7-ylenevinylene-co-alt-1,4-phenylene dibromide] (PFV) was hybridized with Ag@SiO(2) NPs via simple self-assembly procedure, and given high stability, monodispersity. The fluorescence intensity of PFV after assembling on Ag@SiO(2) core-shell NPs is enhanced 1.3-fold compared with the fluorescence intensity of PFV assembled on silica NPs without silver cores for the MEF property of the Ag@SiO(2) nanostructure. Nanocomposite with bright fluorescence was obtained. Moreover, the nanocomposition exhibits good monodispersity and low cytotoxicity, which promote their application in cellular imaging. Furthermore, fluorescent nanoparticles with amendable peripheral surfaces can also be potentially obtained because of the easy modification property of CPs and give potential application in selective biological sensing and imaging.
一种新型荧光纳米粒子是通过基于水溶性共轭聚合物（CPs）和 Ag@SiO(2)核壳纳米粒子的简单自组装技术制备的。具有银 NPs 核的核壳纳米粒子具有一种称为金属增强荧光（MEF）的独特性质。在本工作中，通过简单的自组装程序将阳离子共轭聚合物聚[9,9'-双（6''-（N，N，N-三甲基铵）-己基）芴-2,7-亚基乙烯基-co-alt-1,4-亚苯基二溴化物]（PFV）与 Ag@SiO(2) NPs 杂交，得到了高稳定性、单分散性。与没有银核的二氧化硅 NPs 组装后的 PFV 的荧光强度相比，组装在 Ag@SiO(2)核壳 NPs 上的 PFV 的荧光强度增强了 1.3 倍，这是由于 Ag@SiO(2) 纳米结构的 MEF 性质。得到了具有明亮荧光的纳米复合材料。此外，由于 CPs 易于修饰的特性，该纳米复合材料还表现出良好的单分散性和低细胞毒性，这促进了它们在细胞成像中的应用。此外，由于 CPs 易于修饰的特性，还可以得到具有可修饰的外围表面的荧光纳米粒子，这为选择性生物传感和成像提供了潜在的应用。
