鉴定8α,25-二羟基-9,10-开环-4,6,10(19)-胆甾三烯-3-酮为肝脏微粒体对25-羟基胆钙化醇代谢的产物。

Identification of 8 alpha,25-dihydroxy-9,10-seco-4,6,10(19)-cholestatrien-3-one as a product of metabolism of 25-hydroxycholecalciferol by liver microsomes.

作者信息

Thierry-Palmer M, Richardson K A, Schnoes H K, Yamada S, Free A L

机构信息

Department of Biochemistry, Morehouse School of Medicine, Atlanta, Georgia 30310.

出版信息

Arch Biochem Biophys. 1990 Mar;277(2):277-82. doi: 10.1016/0003-9861(90)90579-n.

DOI:10.1016/0003-9861(90)90579-n

PMID:2310195

Abstract

The ability of liver microsomes, sites of synthesis of 25-hydroxycholecalciferol, to further metabolize 25-hydroxycholecalciferol has been assessed. When liver microsomes were incubated with 25-hydroxycholecalciferol in the presence of cytosol, a metabolite was isolated that comigrated with 8 alpha,25-dihydroxy-9,10-seco-4,6,10(19)-cholestatrien-3- one in three different chromatographic systems. The ultraviolet spectrum (220-350 nm) and mass spectrum of the purified metabolite were identical to that of synthetic 8 alpha,25-dihydroxy-9,10-seco-4,6,10(19)-cholestatrien-3-one. This study indicates that liver microsomes convert 25-hydroxycholecalciferol to 8 alpha,25-dihydroxy-9,10-seco-4,6,10(19)-cholestatrien-3-one. The significance of this metabolite, which has been shown previously by others to be produced by alveolar macrophages, has yet to be determined.

摘要

肝脏微粒体作为25-羟胆钙化醇的合成场所，其进一步代谢25-羟胆钙化醇的能力已得到评估。当肝脏微粒体在胞液存在的情况下与25-羟胆钙化醇一起温育时，分离出了一种代谢产物，该代谢产物在三种不同的色谱系统中与8α,25-二羟基-9,10-开环-4,6,10(19)-胆甾三烯-3-酮一同迁移。纯化代谢产物的紫外光谱（220 - 350纳米）和质谱与合成的8α,25-二羟基-9,10-开环-4,6,10(19)-胆甾三烯-3-酮相同。本研究表明，肝脏微粒体将25-羟胆钙化醇转化为8α,25-二羟基-9,10-开环-4,6,10(19)-胆甾三烯-3-酮。这种代谢产物的意义尚未确定，此前其他人已证明其由肺泡巨噬细胞产生。