将 IRIS iQ200 工作站用作进行尿液培养的屏幕。
IRIS iQ200 workstation as a screen for performing urine culture.
机构信息
Medical Care Line (Infectious Disease Section), Michael E. DeBakey Veterans Affairs Medical Center, Houston, TX 77030, USA.
出版信息
Diagn Microbiol Infect Dis. 2013 Jan;75(1):5-8. doi: 10.1016/j.diagmicrobio.2012.08.026. Epub 2012 Oct 24.
We cultured 1000 urines and tested them with the iQ200 Workstation (Iris Diagnostics, Chatsworth, CA, and Arkray, Edina, MN, USA) for leukocyte esterase and nitrite (LE/N), white blood cells (WBC), all small particles (ASP), bacteria, and yeast. Positive culture grew ≥ 10(4) CFU/mL of 1 or 2 uropathogens. Method A assessed LE/N, WBCs ≥ 6/μL, and bacteria/yeast. Method B assessed WBCs ≥ 6/μL, bacteria/yeast, and ASP ≥ 10,000. Each was compared to culture results, clinician's diagnosis (CD), or expert review (ER). Sensitivities by culture were 88.7% (Method A) and 88.3% (Method B), with negative predictive values (NPVs) of 94.4% and 94.2%, respectively. Sensitivity and NPV increased for comparison to the CD (99.6%) and NPVs of 99.0% and 99.3% compared to ER. ASP did not enhance specificity, sensitivity, or NPV. The iQ200 Workstation performed well by any standard, thus providing a reliable system by which to improve the use of laboratory resources.
我们培养了 1000 份尿液样本，并使用 iQ200 工作站（美国加利福尼亚州恰茨沃斯的 Iris Diagnostics 和明尼苏达州伊迪纳的 Arkray）对白细胞酯酶和亚硝酸盐（LE/N）、白细胞（WBC）、所有小颗粒（ASP）、细菌和酵母进行了检测。阳性培养物的 1 或 2 种尿路病原体的生长≥10(4)CFU/mL。方法 A 评估 LE/N、WBCs≥6/μL 和细菌/酵母。方法 B 评估 WBCs≥6/μL、细菌/酵母和 ASP≥10,000。每种方法均与培养结果、临床医生诊断（CD）或专家审查（ER）进行了比较。根据培养结果，方法 A 的灵敏度为 88.7%，方法 B 的灵敏度为 88.3%，阴性预测值（NPV）分别为 94.4%和 94.2%。与 CD 相比，灵敏度和 NPV 均有所提高（99.6%），与 ER 相比，NPV 分别为 99.0%和 99.3%。ASP 并未提高特异性、灵敏度或 NPV。无论采用何种标准，iQ200 工作站的性能都非常出色，因此提供了一种可靠的系统，可以提高实验室资源的利用效率。
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