Zacharko R M, Kasian M, Irwin J, Zalcman S, LaLonde G, MacNeil G, Anisman H
Carleton University, Psychology Department, Ottawa, Ont., Canada.
Behav Brain Res. 1990 Jan 22;36(3):251-81. doi: 10.1016/0166-4328(90)90062-j.
A behavioral analysis of intracranial self-stimulation (ICSS) was provided for mesolimbic/mesocortical, nigrostriatal, hypothalamic and extrahypothalamic sites in the CD-1 mouse. Robust responding and rapid acquisition of mesocortical ICSS appeared dorsally along notably fluorescent sites in rostral and caudal planes. ICSS was diminished demonstrably in medial and ventral positions in posterior planes. Mesolimbic ICSS from the medial and ventral nucleus accumbens (Nas), was accompanied by significant elevations in locomotor activity, corresponding to regions of dopamine (DA) and cholecystokinin co-localization. Stimulation-induced seizures appeared from both the Nas as well as the mesocortex. ICSS from the ventral tegmental field (VTA) was evident along its medial, lateral and dorsal borders with longer pulse durations more likely to elicit responding. Seizure activity was absent from the VTA. Striatal ICSS was conspicuously poor in dorsal and medial locations; regions presumably devoid of tegmental innervation. ICSS emerged from both the ventrocaudal and anteromedial striatum; regions linked to innervation by the dorsolateral and ventromedial VTA. The red nucleus, a previously neglected self-stimulation site supported marked responding for ICSS. Regions supporting rubral ICSS were correlated with thalamic innervation sites; notably the ventrolateral thalamic nucleus and the parafascicular nucleus, regions found to support ICSS. The substantia nigra supported high rates of responding for ICSS when electrode placement was restricted to the dorsomedial portion of the pars compacta. Electrode deviations lateral and dorsal to the substantia nigra pars medialis induced a progressive decline in responding. Hypothalamic sites were found to support significant responding for ICSS, although such performance was frequently associated with seizure induction. Taken together these data (1) provide the first behavioral analysis of ICSS in mice responding from previously unexamined DA sites in the mesolimbic (e.g. VTA, Nas) and nigrostriatal systems (e.g. caudate, red nucleus) (2) suggest an anatomical reconsideration of the assumptions underlying the elicitation of ICSS from the frontal cortex (3) suggest that the neural circuitry underlying thalamic, caudate, rubral and frontal cortical ICSS are interrelated and (4) suggest that the Nas and the frontal cortex, like the hypothalamus, in the mouse appear to be particularly sensitive to stimulation-induced seizures.
对CD-1小鼠中脑边缘/中脑皮质、黑质纹状体、下丘脑和下丘脑外区域的颅内自我刺激（ICSS）进行了行为分析。中脑皮质ICSS的强烈反应和快速习得出现在沿前后平面显著荧光部位的背侧。在后平面的内侧和腹侧位置，ICSS明显减弱。来自内侧和腹侧伏隔核（Nas）的中脑边缘ICSS伴随着运动活动的显著增加，这与多巴胺（DA）和胆囊收缩素共定位区域相对应。刺激诱发的癫痫发作既出现在Nas，也出现在中脑皮质。来自腹侧被盖区（VTA）的ICSS在其内侧、外侧和背侧边界明显，较长的脉冲持续时间更有可能引发反应。VTA未出现癫痫活动。纹状体ICSS在背侧和内侧位置明显较差；这些区域可能没有被盖神经支配。ICSS出现在尾状核的腹尾侧和前内侧；这些区域与背外侧和腹内侧VTA的神经支配有关。红核，一个以前被忽视的自我刺激部位，对ICSS表现出明显的反应。支持红核ICSS的区域与丘脑神经支配部位相关；特别是腹外侧丘脑核和束旁核，这些区域也支持ICSS。当电极放置限制在致密部的背内侧部分时，黑质对ICSS表现出高反应率。电极向黑质内侧部的外侧和背侧偏移会导致反应逐渐下降。下丘脑部位被发现对ICSS有显著反应，尽管这种表现经常与癫痫发作诱导有关。综合这些数据：（1）首次对小鼠中脑边缘（如VTA、Nas）和黑质纹状体系统（如尾状核、红核）中以前未研究的多巴胺部位的ICSS进行了行为分析；（2）建议对额叶皮质诱发ICSS的潜在假设进行解剖学重新考虑；（3）表明丘脑、尾状核、红核和额叶皮质ICSS的神经回路是相互关联的；（4）表明小鼠中的Nas和额叶皮质，与下丘脑一样，似乎对刺激诱发的癫痫发作特别敏感。
