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Genotypic assessment of isoniazid and rifampin resistance in Mycobacterium tuberculosis: a blind study at reference laboratory level.结核分枝杆菌中异烟肼和利福平耐药性的基因分型评估：参考实验室水平的一项盲法研究
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Differentiation of bacterial 16S rRNA genes and intergenic regions and Mycobacterium tuberculosis katG genes by structure-specific endonuclease cleavage.通过结构特异性核酸内切酶切割对细菌16S rRNA基因、基因间区域及结核分枝杆菌katG基因进行鉴别
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Molecular mechanisms of drug resistance in Mycobacterium tuberculosis.结核分枝杆菌耐药性的分子机制
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Antimicrobial agent resistance in mycobacteria: molecular genetic insights.分枝杆菌中的抗菌药物耐药性：分子遗传学见解
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Mutations in the catalase-peroxidase gene from isoniazid-resistant Mycobacterium tuberculosis isolates.来自耐异烟肼结核分枝杆菌分离株的过氧化氢酶-过氧化物酶基因突变。
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Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis.结核分枝杆菌中利福平耐药性突变的检测
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Molecular basis of streptomycin resistance in Mycobacterium tuberculosis: alterations of the ribosomal protein S12 gene and point mutations within a functional 16S ribosomal RNA pseudoknot.结核分枝杆菌中链霉素耐药性的分子基础：核糖体蛋白S12基因的改变及功能性16S核糖体RNA假结内的点突变
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Tuberculosis in the AIDS era.艾滋病时代的结核病
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Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay.通过聚合酶链反应（PCR）和线性探针分析法快速检测肺结核患者痰液和活检标本中的利福平耐药性。
Tuber Lung Dis. 1995 Oct;76(5):425-30. doi: 10.1016/0962-8479(95)90009-8.
10
PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA.聚合酶链反应-单链构象多态性分析：一种用于检测基因组DNA突变的简单且灵敏的方法。
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通过单链构象多态性和核酸酶切片段长度多态性鉴定耐药结核分枝杆菌

Identification of drug-resistantMycobacterium tuberculosis by single strand conformation polymorphism and cleavase fragment length polymorphism.

作者信息

Sharma M, Altamirano M

机构信息

Vancouver General Hospital and Health Sciences, Vancouver, B.C. Canada.

出版信息

Indian J Clin Biochem. 2000 Jul;15(2):88-93. doi: 10.1007/BF02883734.

DOI:10.1007/BF02883734
PMID:23105247
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3453957/
Abstract

Twenty isolates ofMycobacterium tuberculosis resistant to rifampicin(RIF), isoniazid(INH) and streptomycin(STR) were analysed by Polymerase Chain Reaction (PCR) amplification of rpoB, katG and rrs genes to evaluate comparative diagnostic significance of genetic assays. Mutations were identified by single strand conformation polymorphism (SSCP) and cleavase fragment length polymorphism (CFLP) and were confirmed by DNA sequencing. SSCP of 4 RIF resistant and 14 INH resistant isolates showed an extra peak at the level of 75-bp and 85-bp respectively, while 2 STR resistant isolates showed 2 peaks with 9 bases difference. CFLP showed a different pattern among RIF, INH and STR sensitive and resistant isolates Thus SSCP and CFLP can be used as alternative diagnostic methods for identification of mutations in RIF, INH and STR resistant strains of M.tuberculosis.

摘要

对20株耐利福平（RIF）、异烟肼（INH）和链霉素（STR）的结核分枝杆菌分离株进行了rpoB、katG和rrs基因的聚合酶链反应（PCR）扩增分析，以评估基因检测的比较诊断意义。通过单链构象多态性（SSCP）和裂解酶片段长度多态性（CFLP）鉴定突变，并通过DNA测序进行确认。4株耐RIF分离株和14株耐INH分离株的SSCP分别在75 bp和85 bp水平显示一个额外峰，而2株耐STR分离株显示两个相差9个碱基的峰。CFLP在RIF、INH和STR敏感及耐药分离株中显示出不同模式。因此，SSCP和CFLP可作为鉴定结核分枝杆菌耐RIF、INH和STR菌株突变的替代诊断方法。