De Beenhouwer H, Lhiang Z, Jannes G, Mijs W, Machtelinckx L, Rossau R, Traore H, Portaels F
Department of Microbiology, O. L. V. Hospital, Aalst, Belgium.
Tuber Lung Dis. 1995 Oct;76(5):425-30. doi: 10.1016/0962-8479(95)90009-8.
Multidrug resistant Mycobacterium tuberculosis strains are threatening TB control in the world. Rapid diagnosis of resistance is essential for adequate treatment and optimal control of the disease.
Evaluation of a new technique (Line Probe Assay, LiPA) for easy and rapid detection of Rifampicin resistance (RMPR) of M. tuberculosis.
After amplification of the region of the RNA polymerase, involved in RMPR, the amplified product is hybridized with a set of 10 oligonucleotides immobilized onto a membrane strip. From the pattern obtained the presence or absence of RMPR M. tuberculosis can be assessed. 67 clinical samples positive in culture for M. tuberculosis were analyzed with LiPA and results were compared with classical susceptibility testing.
In vitro drug sensitivity testing identified 46 rifampicin sensitive and 21 resistant strains. In 65 of the 67 specimens LiPA results matched classical testing. In two RMPR cases LiPA showed a sensitive pattern.
In contrast to culture and sensitivity testing, where results take on average 6 weeks, LiPA testing is an easy and rapid (< 48 h) method of detecting RMPR M. tuberculosis in clinical samples. Results correlated in 97% of the samples. In the two RMPR samples with a sensitive LiPA pattern another mechanism of resistance is suspected.
耐多药结核分枝杆菌菌株正威胁着全球结核病的控制。快速诊断耐药性对于疾病的充分治疗和最佳控制至关重要。
评估一种用于简便快速检测结核分枝杆菌利福平耐药性（RMPR）的新技术（线性探针测定法，LiPA）。
在扩增参与RMPR的RNA聚合酶区域后，将扩增产物与固定在膜条上的一组10种寡核苷酸杂交。根据获得的模式可以评估结核分枝杆菌RMPR的存在与否。用LiPA分析了67份结核分枝杆菌培养阳性的临床样本，并将结果与经典药敏试验进行比较。
体外药物敏感性试验鉴定出46株利福平敏感菌株和21株耐药菌株。67份标本中有65份LiPA结果与经典试验相符。在两例RMPR病例中，LiPA显示出敏感模式。
与平均需要6周才能得出结果的培养和药敏试验不同，LiPA检测是一种简便快速（<48小时）的检测临床样本中结核分枝杆菌RMPR的方法。97%的样本结果相关。在两例LiPA模式呈敏感的RMPR样本中，怀疑存在另一种耐药机制。
