Basu P S, Majhi R, Chatterjee S, Batabyal S K
Indian Institute of Chemical Biology, 4 Raja S C Mullick Road, 700032 Calcutta.
Indian J Clin Biochem. 2000 Jul;15(2):119-23. doi: 10.1007/BF02883739.
A method has been developed to separate and identify thyroglobulin autoantibody (TgAb) and thyroid peroxidase autoantibody (TPOAb) in serum obtained from normal and autoimmune thyroid diseases using phenyl Sepharose CL-4B hydrophobic column. The protein peaks obtained from hydrophobic column were identified as TgAb and TPOAb by comparing the elution profile of commercially purified standard thyroid autoantibodies. The similarity of the inhibitory effects of eluted proteins and of standard thyroid autoantibodies on lectin concanavalin A-RBC interaction confirmed the separation of TPO-Ab and TgAb by the hydrophobic, column. The eluted fractions from the hydrophobic column were estimated by the radio immunoassay (RIA) to confirm the presence of both auto-antibodies. This hydrophobic column method offers an advantage of visual inspection of this autoantibodies by graphic representation of peak height along with their estimation in autoimmune thyroid disorders.
已开发出一种方法，使用苯基琼脂糖凝胶CL - 4B疏水柱，从正常人和自身免疫性甲状腺疾病患者的血清中分离并鉴定甲状腺球蛋白自身抗体（TgAb）和甲状腺过氧化物酶自身抗体（TPOAb）。通过比较市售纯化标准甲状腺自身抗体的洗脱图谱，确定从疏水柱获得的蛋白质峰为TgAb和TPOAb。洗脱蛋白与标准甲状腺自身抗体对凝集素伴刀豆球蛋白A - 红细胞相互作用的抑制作用相似，证实了通过疏水柱分离TPO - Ab和TgAb。通过放射免疫测定（RIA）对疏水柱的洗脱级分进行评估，以确认两种自身抗体的存在。这种疏水柱方法具有优势，通过绘制峰高的图形表示以及在自身免疫性甲状腺疾病中的评估，可直观检测这些自身抗体。
