Sachidhanandam M, Singh S N, Salhan A K, Ray Uday Sankar
Defence Institute of Physiology and Allied Sciences, Delhi, 110054 India.
Indian J Clin Biochem. 2010 Apr;25(2):153-7. doi: 10.1007/s12291-010-0028-8. Epub 2010 May 27.
The study involved three ethnic groups of India; Rajputs, Gorkhas and South-Indians. Each group consisted of ∼40 healthy, male soldiers between 20-50 years. The reference ranges for cortisol, testosterone, prolactin, arginine vasopressin and proAtrial natriuretic peptide(1-98) were determined using Enzyme-Immunoassay (EIA) while plasma levels of thyroid-stimulating hormone, triiodothyronine, free-triiodothyronine, thyroxine and freethyroxine were measured using Enzyme-linked immunosorbent assay (ELISA). The results indicated that plasma hormone concentrations were within physiological range and inter-ethnic differences were most prominent between north- (Rajputs and Gorkhas) and south- Indians. In comparison to Radioimmunoassay, the EIA method for prolactin, thyroid-stimulating hormone, free-thyroxine gave higher values while the ELISA method for triiodothyronine, free-triiodothyronine, and thyroxine gave lower values. These differences are due to differences in assay standards and design.
拉杰普特人、廓尔喀人以及南印度人。每个族群由约40名年龄在20至50岁之间的健康男性士兵组成。使用酶免疫分析法（EIA）测定皮质醇、睾酮、催乳素、精氨酸加压素和心钠素原（1-98）的参考范围，而使用酶联免疫吸附测定法（ELISA）测量促甲状腺激素、三碘甲状腺原氨酸、游离三碘甲状腺原氨酸、甲状腺素和游离甲状腺素的血浆水平。结果表明，血浆激素浓度在生理范围内，并且北方（拉杰普特人和廓尔喀人）与南印度人之间的族群差异最为显著。与放射免疫分析法相比，EIA法测定催乳素、促甲状腺激素、游离甲状腺素时得出的值更高，而ELISA法测定三碘甲状腺原氨酸、游离三碘甲状腺原氨酸和甲状腺素时得出的值更低。这些差异是由于测定标准和设计的不同所致。
