Department of Orthopaedic Surgery, CHA Bundang Medical Center, CHA University, Gyeonggi-do, 463-712, Korea.
J Inflamm (Lond). 2012 Oct 30;9(1):40. doi: 10.1186/1476-9255-9-40.
The purpose of this study was to determine the reaction mechanism of corticosteroid by analyzing the expression patterns of neuropeptides (substance P (SP), calcitonin gene related peptide (CGRP)) and of cytokines (interleukin (IL)-1α, tumor growth factor (TGF)-β) after corticosteroid treatment in lateral epicondylitis. In addition, we also investigated whether corticosteroid influenced tenocyte viability.
The corticosteroid triamcinolone acetonide (TAA) was applied to cultured tenocytes of lateral epicondylitis, and the changes in the mRNA expressions of neuropeptides and cytokines and tenocyte viabilities were analyzed at seven time points. Quantitative real-time polymerase chain reaction and an MTT assay were used.
The expression of SP mRNA was maximally inhibited by TAA at 24 hours but recovered at 72 hours, and the expressions of CGRP mRNA and IL-1α mRNA were inhibited at 24 and 3 hours, respectively. The expression of TGF-β mRNA was not significant. Tenocyte viability was significantly reduced by TAA at 24 hours.
We postulate that the reaction mechanism predominantly responsible for symptomatic relief after a corticosteroid injection involves the inhibitions of neuropeptides and cytokines, such as, CGRP and IL-1α. However the tenocyte viability was compromised by a corticosteroid.
本研究旨在通过分析外侧髁炎皮质类固醇治疗后神经肽（P 物质（SP）、降钙素基因相关肽（CGRP））和细胞因子（白细胞介素（IL）-1α、肿瘤生长因子（TGF）-β）表达模式，确定皮质类固醇的反应机制。此外，我们还研究了皮质类固醇是否影响腱细胞活力。
将皮质类固醇曲安奈德（TAA）应用于外侧髁炎的培养腱细胞，并在七个时间点分析神经肽和细胞因子的 mRNA 表达变化和腱细胞活力。采用定量实时聚合酶链反应和 MTT 测定法。
TAA 可在 24 小时内最大程度地抑制 SP mRNA 的表达，但在 72 小时时恢复，CGRP mRNA 和 IL-1α mRNA 的表达分别在 24 小时和 3 小时时受到抑制，TGF-β mRNA 的表达无明显变化。TAA 在 24 小时时显著降低腱细胞活力。
我们推测，皮质类固醇注射后症状缓解的主要反应机制涉及神经肽和细胞因子（如 CGRP 和 IL-1α）的抑制。然而，皮质类固醇会损害腱细胞活力。
