大鼠载脂蛋白B在溶解性方面与人载脂蛋白B不同。

Rat apolipoprotein B differs in solubility properties from human apolipoprotein B.

作者信息

Kamanna V S, Stiers D L, Lee D M

机构信息

Lipoprotein and Atherosclerosis Research Program, Oklahoma Medical Research Foundation, Oklahoma City 73104.

出版信息

Biochim Biophys Acta. 1990 Mar 12;1043(1):119-21. doi: 10.1016/0005-2760(90)90119-i.

DOI:10.1016/0005-2760(90)90119-i

PMID:2310757

Abstract

Apolipoprotein B (ApoB) in general is known as an insoluble protein in aqueous buffers without the initial aid of denaturing agents. However, following the total delipidization of rat plasma low-density lipoproteins, a considerable portion (28.2 +/- 3.0%) of rat ApoB was found directly soluble in an aqueous buffer, N-ethylmorpholine acetate (pH 7.3) as demonstrated by SDS-polyacrylamide gel electrophoresis, immunoblotting and electron microscopic analysis. On the other hand, this was not observed for human ApoB. This solubility difference may suggest some structural differences that exist between rat ApoB and human ApoB.

摘要

一般来说，载脂蛋白B（ApoB）在没有变性剂初步辅助的情况下，是一种在水性缓冲液中不溶的蛋白质。然而，在大鼠血浆低密度脂蛋白完全脱脂后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、免疫印迹和电子显微镜分析表明，相当一部分（28.2±3.0%）的大鼠ApoB可直接溶于水性缓冲液N-乙基吗啉乙酸盐（pH 7.3）中。另一方面，人ApoB并未观察到这种情况。这种溶解性差异可能表明大鼠ApoB和人ApoB之间存在一些结构差异。