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氨酰转移核糖核酸合成酶的亲和层析。同源转移核糖核酸作为配体。

Affinity chromatography of aminoacyl-transfer ribonucleic acid synthetases. Cognate transfer ribonucleic acid as a ligand.

作者信息

Clarke C M, Knowles J R

出版信息

Biochem J. 1977 Nov 1;167(2):419-28. doi: 10.1042/bj1670419.

DOI:10.1042/bj1670419
PMID:23108
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1183673/
Abstract

The use of tRNA affinity columns for the purification of aminoacyl-tRNA synthetases was investigated. A purification method for valyl-tRNA synthetase from Bacillus stearothermophilus is described that uses two affinity columns, one containing the pure cognate tRNA, and the other containing all tRNA species except the cognate tRNA. A method for the rapid preparation of the two columns was developed, which does not require prior isolation of cognate tRNA but makes use of the ability of the target synthetase to select its cognate tRNA. The usefulness of tRNA columns is compared with that of affinity columns derived from the aminoalkyladenylate reported in the preceding paper [Clarke & Knowles (1977) Biochem J. 167, 405-417].

摘要

研究了使用tRNA亲和柱纯化氨酰-tRNA合成酶的方法。描述了一种从嗜热脂肪芽孢杆菌中纯化缬氨酰-tRNA合成酶的方法，该方法使用两个亲和柱，一个含有纯同源tRNA，另一个含有除同源tRNA之外的所有tRNA种类。开发了一种快速制备这两种柱子的方法，该方法不需要预先分离同源tRNA，而是利用目标合成酶选择其同源tRNA的能力。将tRNA柱的实用性与前文[Clarke & Knowles (1977) Biochem J. 167, 405 - 417]报道的氨基烷基腺苷酸衍生的亲和柱的实用性进行了比较。

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引用本文的文献

1
Affinity chromatography of aminoacyl-transfer ribonucleic acid synthetases. Small organic ligands.氨酰转移核糖核酸合成酶的亲和层析。小分子有机配体。
Biochem J. 1977 Nov 1;167(2):405-17. doi: 10.1042/bj1670405.

本文引用的文献

1
PARTIAL PURIFICATION OF SOLUBLE RNA.可溶性核糖核酸的部分纯化
Proc Natl Acad Sci U S A. 1960 Jun;46(6):811-22. doi: 10.1073/pnas.46.6.811.
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DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
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Purification of yeast phenylalanyl-tRNA synthetase by affinity chromatography, on a tRNA(Phe)-sepharose column.通过在tRNA（苯丙氨酸）-琼脂糖柱上进行亲和层析来纯化酵母苯丙氨酰-tRNA合成酶。
FEBS Lett. 1972 Oct 15;27(1):134-138. doi: 10.1016/0014-5793(72)80426-5.
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Studies on polynucleotides. LXXVII. The labeling of end groups in polynucleotide chains: the selective modification of diol end groups in ribonucleic acids.多核苷酸研究。第七十七部分。多核苷酸链末端基团的标记：核糖核酸中二醇末端基团的选择性修饰。
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Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
J Biol Chem. 1970 Jun;245(12):3059-65.
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Application of affinity chromatography to amino-acyl-ribonucleic acid synthetase. I.亲和色谱法在氨酰核糖核酸合成酶上的应用。I.
J Biochem. 1973 Aug;74(2):203-8.
9
Relation between aminoacyl-tRNA stability and the fixed amino acid.氨酰-tRNA稳定性与固定氨基酸之间的关系。
Biochim Biophys Acta. 1972 Oct 11;281(2):228-32. doi: 10.1016/0005-2787(72)90174-8.
10
A comparison of purified valyl-transfer ribonucleic acid synthetase from Bacillus stearothermophilus and from Escherichia coli.嗜热脂肪芽孢杆菌和大肠杆菌中纯化的缬氨酰转移核糖核酸合成酶的比较。
Biochem J. 1974 May;139(2):391-8. doi: 10.1042/bj1390391.

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