De Pedro Pedro, Wilkins Pamela A, McMichael Maureen A, Dirikolu Levent, Lascola Kara M, Clark-Price Stuart C, Boston Raymond C
Department of Veterinary Clinical Medicine, University of Illinois at Urbana-Champaign, 1008W. Hazelwood Drive, Urbana, IL 61802, USA.
J Vet Emerg Crit Care (San Antonio). 2012 Oct;22(5):564-72. doi: 10.1111/j.1476-4431.2012.00800.x.
To determine endogenous production of L-lactate and the clearance of exogenous sodium L-lactate (ExLC) in healthy adult horses.
A sodium L-lactate solution (1 mmol/kg body weight qs to 500 mL final volume in 0.9% NaCl) was adminstered IV over 15 minutes. Blood samples for L-lactate concentration [LAC] measurement were collected immediately prior to infusion, at 5, 10, and 15 minutes during infusion and at 1 minute intervals for 15 minutes, at 30, 45, 60, 120, and 180 minutes postinfusion. Disposition modeling and pharmacokinetic analysis was performed using proprietary software.
University Teaching Hospital.
Six clinically healthy adult horses.
Median (range) baseline [LAC] was 0.43 (0.20-0.72) mmol/L for samples obtained every 3 hours over the 24 hours prior to ExLC and demonstrated variability primarily associated with horse. Median [LAC] immediately prior to ExLC was 0.43 (0.35-0.52) mmol/L. A 2-compartment model was used to specify the pharmacokinetic parameters. Median (range) ExLC was 1.05 (0.073-1.75) L·h(-1) ·kg(-1) and t(1/2) β was 29.54 (20.8-38.6) min. Median lactate production based on basal [LAC] immediately prior to ExLC was was 0.49 (0.31-0.93) mmol·h(-1) ·kg(-1) .
ExLC in healthy adult horses is greater than that of hyperlactemic human patients but similar to normolactemic-sick human patients examined using the same model, supporting development of species, and disease specific ExLC parameters.
测定健康成年马体内L-乳酸的内源性生成及外源性L-乳酸钠（ExLC）的清除率。
静脉输注L-乳酸钠溶液（1 mmol/kg体重，以0.9%氯化钠溶液定容至最终体积500 mL），输注时间为15分钟。在输注前即刻、输注过程中的第5、10和15分钟以及输注后1分钟间隔共15分钟、输注后30、45、60、120和180分钟采集血样测定L-乳酸浓度[LAC]。使用专用软件进行处置建模和药代动力学分析。
大学教学医院。
6匹临床健康的成年马。
在给予ExLC前24小时内，每间隔3小时采集的样本中，[LAC]中位数（范围）为0.43（0.20 - 0.72）mmol/L，且主要与马匹个体差异有关。给予ExLC前即刻[LAC]中位数为0.43（0.35 - 0.52）mmol/L。采用二室模型确定药代动力学参数。ExLC中位数（范围）为1.05（0.073 - 1.75）L·h⁻¹·kg⁻¹，t₁/₂β为29.54（20.8 - 38.6）分钟。基于给予ExLC前即刻基础[LAC]的乳酸生成中位数为0.49（0.31 - 0.93）mmol·h⁻¹·kg⁻¹。
健康成年马的ExLC高于高乳酸血症的人类患者，但与使用相同模型检测的正常乳酸血症患病人类患者相似，这支持了针对不同物种和疾病制定特定的ExLC参数。
