Yazdani M, Joseph F, Grant S, Hartman A D, Nakamoto T
Laboratory of Perinatal Nutrition and Metabolism, Louisiana State University Medical Center, New Orleans.
Dev Pharmacol Ther. 1990;14(1):52-61.
Pregnant dams were divided into four groups on day 10 of gestation. Dams of group 1 were fed an 20% protein diet as controls. Dams of groups 2, 3 and 4 were fed a 20% protein diet supplemented with 0.5 mg, 1 mg and 2 mg caffeine/100 g body weight of dams, respectively. Pups were delivered surgically on day 22, and their brains were rapidly removed and analyzed for DNA, protein, cholesterol, zinc and alkaline phosphatase activity. The dams' brains were analyzed for the same parameters as those of the pups. Plasma and brain caffeine levels were also determined in caffeine-supplemented groups. The pups' brains in group 2 were heavier than those in group 4. The DNA concentration of group 2 was higher than that of the other groups. The protein concentration of group 4 was higher than that of the other groups. The cholesterol concentration of group 3 and 4 was less than that of the controls. The zinc concentration of group 4 was less than that of group 2. Alkaline phosphatase activity was decreased in groups 3 and 4 compared with either controls or group 2. Dams showed no significant difference among the groups in the same biochemical parameters except for cholesterol concentration that was higher in groups 2, 3 and 4 than in the controls. Plasma and brain caffeine levels of the fetuses and plasma caffeine of the dams in group 4 were higher than those of either group 2 or 3. It is concluded that various amounts of maternal caffeine intake exert different effects on fetal brain growth. In contrast, the effect of caffeine on the dams' brain is relatively minor.
妊娠母鼠在妊娠第10天被分为四组。第1组母鼠喂食20%蛋白质饮食作为对照。第2、3和4组母鼠分别喂食添加了0.5毫克、1毫克和2毫克咖啡因/100克母鼠体重的20%蛋白质饮食。在第22天通过手术分娩幼崽，迅速取出它们的大脑并分析其DNA、蛋白质、胆固醇、锌和碱性磷酸酶活性。对母鼠的大脑进行与幼崽相同参数的分析。还测定了补充咖啡因组的血浆和大脑咖啡因水平。第2组幼崽的大脑比第4组的重。第2组的DNA浓度高于其他组。第4组的蛋白质浓度高于其他组。第3组和第4组的胆固醇浓度低于对照组。第4组的锌浓度低于第2组。与对照组或第2组相比，第3组和第4组的碱性磷酸酶活性降低。除了第2、3和4组的胆固醇浓度高于对照组外，母鼠在相同生化参数上各组间无显著差异。第4组胎儿的血浆和大脑咖啡因水平以及母鼠的血浆咖啡因水平高于第2组或第3组。结论是，母体摄入不同量的咖啡因对胎儿大脑生长产生不同影响。相比之下，咖啡因对母鼠大脑的影响相对较小。
