2-脱氧-D-半乳糖，大肠杆菌半乳糖转运系统的一种底物。
2-Deoxy-D-galactose, a substrate for the galactose-transport system of Escherichia coli.
作者信息
Henderson P J, Giddens R A
出版信息
Biochem J. 1977 Oct 15;168(1):15-22. doi: 10.1042/bj1680015.
The following observations showed that 2-deoxy-D-galactose is a useful tool for the isolation and elucidation of the activity of one system for galactose uptake into Escherichia coli. 1. 2-Deoxygalactose, which is not a substrate for growth of E. coli, was transported into strains of the organism induced for galactose transport. 2. By using appropriate mutants it was shown that 2-deoxygalactose is a much better substrate for the galactose-transport system than for the methyl galactoside-transport system. This was confirmed by the results of mutual inhibition studies with substrates of each transport system. 3. The glucose-, arabinose- or lactose-transport systems did not effect significant transport of 2-deoxygalactose. 4. Like other substrates of the galactose-transport system, 2-deoxygalactose promoted effective proton uptake into de-energized suspensions of appropriate E. coli strains. 5. The S183 series of E. coli mutants were found to contain a constitutive galactose-transport system, if 2-deoxygalactose transport is used as one criterion for such activity.
以下观察结果表明，2-脱氧-D-半乳糖是用于分离和阐明大肠杆菌中半乳糖摄取系统活性的有用工具。1. 2-脱氧半乳糖不是大肠杆菌生长的底物，但可被诱导进行半乳糖转运的该生物体菌株摄取。2. 通过使用合适的突变体表明，2-脱氧半乳糖对于半乳糖转运系统而言是比甲基半乳糖苷转运系统更好的底物。这通过各转运系统底物的相互抑制研究结果得到证实。3. 葡萄糖、阿拉伯糖或乳糖转运系统对2-脱氧半乳糖的转运没有显著影响。4. 与半乳糖转运系统的其他底物一样，2-脱氧半乳糖促进了合适的大肠杆菌菌株去能悬浮液中有效的质子摄取。5. 如果将2-脱氧半乳糖转运用作此类活性的一个标准，则发现大肠杆菌S183系列突变体含有组成型半乳糖转运系统。
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